LOCUS       sequence-85427-e        2566 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions/Membrane, Excitation: 587, Emission: 
            610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2566)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Ezrin-N-14
FEATURES             Location/Qualifiers
     source          1..2566
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1821..1830
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1827..2537
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1972..1990)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2240..2259
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gtgacgtcag atccgctagc gccaccatgc cgaaaccaat caatgtccga gttaccacca
       61 tggatgcaga gctggagttt gcaatccagc caaatacaac tggaaaacag ctttttgatc
      121 aggtggtaaa gactatcggc ctccgggaag tgtggtactt tggcctccac tatgtggata
      181 ataaaggatt tcctacctgg ctgaagctgg ataagaaggt gtctgcccag gaggtcagga
      241 aggagaatcc cctccagttc aagttccggg ccaagttcta ccctgaagat gtggctgagg
      301 agctcatcca ggacatcacc cagaaacttt tcttcctcca agtgaaggaa ggaatcctta
      361 gcgatgagat ctactgcccc cctgagactg ccgtgctctt ggggtcctac gctgtgcagg
      421 ccaagtttgg ggactacaac aaagaagtgc acaagtctgg gtacctcagc tctgagcggc
      481 tgatccctca aagagtgatg gaccagcaca aacttaccag ggaccagtgg gaggaccgga
      541 tccaggtgtg gcatgcggaa caccgtggga tgctcaaaga taatgctatg ttggaatacc
      601 tgaagattgc tcaggacctg gaaatgtatg gaatcaacta tttcgagata aaaaacaaga
      661 aaggaacaga cctttggctt ggagttgatg cccttggact gaatatttat gagaaagatg
      721 ataagttaac cccaaagatt ggctttcctt ggagtgaaat caggaacatc tctttcaatg
      781 acaaaaagtt tgtcattaaa cccatcgaca agaaggcacc tgactttgtg ttttatgccc
      841 cacgtctgag aatcaacaag cggatcctgc agctctgcat gggcaaccat gagttgtata
      901 tgcgccgcag gaagcctgac accatcgagg tgcagcagat gaaggcccag gcccgggagg
      961 agaagcatca gaagcagctg gagcggcaac agctggaaac agagaagaaa aggagagaaa
     1021 ccgtggagag agagaaagag cagatgatgc gcgagaagga ggagttgatg ctgcggctgc
     1081 aggactatga ggagaagaca aagaaggcag agagagagct ctcggagcag attcagaggg
     1141 ccctgcagct ggaggaggag aggaagcggg cacaggagga ggccgagcgc ctagaggctg
     1201 accgtatggc tgcactgcgg gctaaggagg agctggagag acaggcggtg gatcagataa
     1261 agagccagga gcagctggct gcggagcttg cagaatacac tgccaagatt gccctcctgg
     1321 aagaggcgcg gaggcgcaag gaggatgaag ttgaagagtg gcagcacagg gccaaagaag
     1381 cccaggatga cctggtgaag accaaggagg agctgcacct ggtgatgaca gcacccccgc
     1441 ccccaccacc ccccgtgtac gagccggtga gctaccatgt ccaggagagc ttgcaggatg
     1501 agggcgcaga gcccacgggc tacagcgcgg agctgtctag tgagggcatc cgggatgacc
     1561 gcaatgagga gaagcgcatc actgaggcag agaagaacga gcgtgtgcag cggcagctgc
     1621 tgacgctgag cagcgagctg tcccaggccc gagatgagaa taagaggacc cacaatgaca
     1681 tcatccacaa cgagaacatg aggcaaggcc gggacaagta caagacgctg cggcagatcc
     1741 ggcagggcaa caccaagcag cgcatcgacg agttcgaggc cctggcatcc ggcggtagcg
     1801 gcggtggcag cggaccggtc gccaccatgg tgagcaaggg cgaggaggat aacatggcca
     1861 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt
     1921 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
     1981 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt
     2041 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct
     2101 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg
     2161 tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca
     2221 ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct
     2281 ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc
     2341 tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg
     2401 tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg
     2461 actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg
     2521 acgagctgta caagtaaagc ggccgcgact ctagatcata atcagc
//
