LOCUS       sequence-85428-e        3976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal adhesion kinase, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3976)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 3976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-FAK-5
FEATURES             Location/Qualifiers
     source          1..3976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..741
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(179..197)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     447..466
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggaggataac
       61 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
      121 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
      181 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
      241 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
      301 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
      361 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
      421 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
      481 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
      541 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
      601 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
      661 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
      721 ggcatggacg agctgtacaa gtccggactc agatctatgg cagcagctta ccttgatcca
      781 aacttgaatc atacaccaag ttcaagtgca aagacgcacc tcggtactgg gatggagcgt
      841 tccccggggg ccatggagcg agtcctaaag gtttttcact actttgaaaa cagcagcgag
      901 ccaacgacgt gggccagcat tatccggcat ggagatgcta ctgatgttcg aggcataata
      961 cagaagattg tggactgtca caaagtgaaa aatgtggcct gctatgggtt gcgactcagt
     1021 catctgcagt ctgaggaggt tcactggctg cacctggaca tgggggtatc caatgtgaga
     1081 gagaaatttg aactagcaca tcctccagaa gaatggaaat atgaactgag aattcggtac
     1141 ctgcccaaag gatttctaaa ccagttcact gaggacaaac caactttaaa ttttttctat
     1201 cagcaggtga aaaatgacta tatgttagaa atagcagatc aagtggacca ggaaattgct
     1261 ttgaaactag gttgccttga aatcaggaga tcctacggag agatgagagg caatgcatta
     1321 gagaagaaat ccaactatga agtgctagaa aaagatgtcg gtttaagacg attttttccg
     1381 aagagtttgc tagattcagt gaaggccaaa acactacgaa aattaatcca acagacattt
     1441 cgacaatttg ccaacctcaa cagagaagaa agtattttga aattctttga gatcctctct
     1501 ccagtgtaca gatttgacaa ggaatgcttc aagtgtgccc ttggttcaag ctggattatt
     1561 tcagtggagc tggcaattgg cccagaggaa ggaatcagct accttacaga caagggtgca
     1621 aatccaactc acctggcaga ttttaatcaa gtacaaacta ttcagtattc aaacagtgaa
     1681 gacaaggaca gaaaagggat gttgcaactg aagatagctg gtgcacctga gcctctgaca
     1741 gtgacagcac catccttaac cattgcagag aatatggctg acttgataga cggatactgc
     1801 cgactggtga atggagccac gcaatctttt attatcaggc cacagaaaga aggtgaaaga
     1861 gctttaccat caataccaaa gctggccaac aatgagaagc aaggagtaag gtcgcacaca
     1921 gtctctgtat cagaaacaga tgactatgca gagataatag atgaagaaga tacttataca
     1981 atgccatcaa ccagagatta tgaaattcaa agggagagaa ttgaactggg gcgctgcatt
     2041 ggtgaaggac agtttggaga tgtgcaccaa ggaatttaca tgagtccgga aaatccagct
     2101 atggctgtag caatcaaaac atgtaaaaac tgcacctcag acagcgttag agaaaagttc
     2161 ctacaagaag ccttaacaat gcgtcagttt gatcatcctc acattgtgaa gctcattgga
     2221 gttattacag aaaacccagt gtggataatc atggagctct gtacacttgg agagttgaga
     2281 tcgtttctgc aagtaagaaa attcagcttg gacctggcct ccctcatcct ctacgcttac
     2341 cagcttagca cagcacttgc ttacctagag agcaaaagat ttgtacatag agatattgct
     2401 gctaggaacg tgctggtatc tgccactgac tgtgtgaaat tgggtgactt tggcttatcc
     2461 cgatacatgg aagacagtac ttactataaa gcttccaaag gaaagttacc tatcaaatgg
     2521 atggctccag agtcaatcaa cttccgacgg tttacctcag caagcgatgt gtggatgttt
     2581 ggtgtgtgta tgtgggagat cctgatgcat ggggtaaagc ccttccaggg agtgaaaaat
     2641 aatgatgtta ttggtcggat tgagaacggt gagcggctcc ccatgcctcc gaactgccct
     2701 cccaccctct acagccttat gaccaagtgc tgggcatacg accctagtag acgacccagg
     2761 tttactgaac ttaaagcaca actcagtaca atactggagg aggagaagct gcagcaagag
     2821 gaacgaatga gaatggaatc caggcgacaa gtcacagtat cctgggactc aggaggatca
     2881 gatgaagctc ctcccaagcc cagcaggcct ggttacccca gcccaaggtc cagtgaaggg
     2941 ttttatccga gtcctcagca tatggtacag ccaaatcact accaggtatc tggctactct
     3001 ggttctcatg ggataccagc catggcaggc agcatttatc ctgggcaagc ttctctcttg
     3061 gatcaaacag attcctggaa ccatcgacct caggaagtat cagcatggca gccaaacatg
     3121 gaggattcgg gcactttgga tgtacgagga atggggcagg ttctgcccac acatctcatg
     3181 gaggagaggt taataagaca acagcaagag atggaagaag atcaacgctg gcttgagaaa
     3241 gaggaacgat tcctggtaat gaaacctgat gtgcggctct ccagaggcag cattgaacgg
     3301 gaggacggag gtctccaggg cccagctggt aaccagcaca tatatcagcc tgtgggtaaa
     3361 ccagatcatg ccgctccacc aaagaagccc cctcgccctg gagcccccca cttgggcagc
     3421 ctcgcgagcc tgaacagccc cgtggacagc tacaacgaag gcgtgaagat caagccacag
     3481 gaaatcagcc ctcctcctac ggccaacctg gaccgctcca atgacaaagt ctatgagaat
     3541 gtaaccgggc tggtgaaagc tgtcatagag atgtccagta aaatacagcc agctccgcca
     3601 gaggagtacg tgcccatggt aaaggaggtt ggcttggcgc tgagaacctt gctagcaaca
     3661 gtggatgagt cgctgccagt gcttcctgca agcacccaca gagagattga gatggcccag
     3721 aaactgctga actctgacct ggctgagctc attaacaaga tgaagctggc ccagcagtac
     3781 gtcatgacca gcctgcagca ggagtacaag aagcaaatgc tgacggctgc tcacgctctg
     3841 gctgtggatg ccaagaactt gctggatgtc atcgatcaag ccagactgaa aatgatcagc
     3901 cagtccaggc ccactaagct gccgtagccg aattctgcag atgggatcca ccggatctag
     3961 ataactgatc ataatc
//
