LOCUS       sequence-85430-e        1775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty backbone for fusing gene of interest to N-term of mKO.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mKusabira Orange-N1
FEATURES             Location/Qualifiers
     source          1..1775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..742
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(180..198)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     448..467
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             complement(1538..1549)
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggaggataa
       61 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
      121 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
      181 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
      241 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
      301 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
      361 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
      421 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
      481 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag
      541 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa
      601 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca
      661 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
      721 cggcatggac gagctgtaca agtccggact cagatctcga gccatgaagc caggattcag
      781 tccccgtggg ggtggctttg gcggccgagg gggctttggt gaccgtggtg gtcgtggagg
      841 ccgagggggc tttggcgggg gccgaggcca aggcggaggc tttagaggtc gtggacgagg
      901 aggaggtgga ggcggcggcg gcggtggagg aggaggaaga ggtggtggag gcttccattt
      961 tggtggcaac cggggtcgtg gtcggggagg aaaaagagga aaccagtcgg ggaagaatgt
     1021 gatggtggag ccgcatcggc atgagggtgt cttcatttgt cggggaaagg aagatgcact
     1081 ggtcaccaag aacctggtcc ctggggaatc agtttatgga gagaagagag tctcgatttc
     1141 ggaaggagat gacaaaattg agtaccgagc ctggaacccc ttccgctcca agctagcagc
     1201 agcaatcctg ggtggtgtgg accagatcca catcaaaccg ggggctaagg ttctctacct
     1261 cggggctgcc tcgggcacca cggtctccca tgtctctgac atcgttggtc cggatggtct
     1321 agtctatgca gtcgagttct cccaccgctc tggccgtgac ctcattaact tggccaagaa
     1381 gaggaccaac atcattcctg tgatcgagga tgctcgacac ccacacaaat accgcatgct
     1441 catcgcaatg gtggatgtga tctttgctga tgtggcccag ccagaccaga cccggattgt
     1501 ggccctgaat gcccacacct tcctgcgtaa tggaggacac tttgtgattt ccattaaggc
     1561 caactgcatt gactccacag cctcagccga ggccgtgttt gcctccgaag tgaaaaagat
     1621 gcaacaggag aacatgaagc cgcaggagca gttgaccctt gagccatatg aaagagacca
     1681 tgccgtggtc gtgggagtgt acaggccacc ccccaaggtg aagaactgag gatccaccgg
     1741 atctagataa ctgatcataa tcagccatac cacat
//
