LOCUS       sequence-85431-e        8721 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin/Focal Adhesions, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8721)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 8721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-FilaminA-N-9
FEATURES             Location/Qualifiers
     source          1..8721
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7966..7975
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7972..8682
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(8117..8135)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8385..8404
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gtgaccgtca gatccgctag cgccaccatg agtagctccc actctcgggc gggccagagc
       61 gcagcaggcg cggctccggg cggcggcgtc gacacgcggg acgccgagat gccggccacc
      121 gagaaggacc tggcggagga cgcgccatgg aagaagatcc agcagaacac tttcacgcgc
      181 tggtgcaacg agcacctgaa gtgcgtgagc aagcgcatcg ccaacctgca gacggacctg
      241 agcgacgggc tgcggcttat cgcgctgttg gaggtgctca gccagaagaa gatgcaccgc
      301 aagcacaacc agcggcccac tttccgccaa atgcagcttg agaacgtgtc ggtggcactc
      361 gagttcctgg accgcgagag catcaaactg gtgtccatcg acagcaaggc catcgtggac
      421 gggaacctga agctgatcct gggcctcatc tggaccctga tcctgcacta ctccatctcc
      481 atgcccatgt gggacgagga ggaggatgag gaggccaaga agcagacccc caagcagagg
      541 ctcctgggct ggatccagaa caagctgccg cagctgccca tcaccaactt cagccgggac
      601 tggcagagcg gccgggccct gggcgccctg gtggacagct gtgccccggg cctgtgtcct
      661 gactgggact cttgggacgc cagcaagccc gttaccaatg cgcgagaggc catgcagcag
      721 gcggatgact ggctgggcat cccccaggtg atcacccccg aggagattgt ggaccccaac
      781 gtggacgagc actctgtcat gacctacctg tcccagttcc ccaaggccaa gctgaagcca
      841 ggggctccct tgcggcccaa actgaacccg aagaaagccc gtgcctacgg gccaggcatc
      901 gagcccacag gcaacatggt gaagaagcgg gcagagttca ctgtggagac cagaagtgct
      961 ggccagggag aggtgctggt gtacgtggag gacccggccg gacaccagga ggaggcaaaa
     1021 gtgaccgcca ataacgacaa gaaccgcacc ttctccgtct ggtacgtccc cgaggtgacg
     1081 gggactcata aggttactgt gctctttgct ggccagcaca tcgccaagag ccccttcgag
     1141 gtgtacgtgg ataagtcaca gggtgacgcc agcaaagtga cagcccaagg tcccggcctg
     1201 gagcccagtg gcaacatcgc caacaagacc acctactttg agatctttac ggcaggagct
     1261 ggcacgggcg aggtcgaggt tgtgatccag gaccccatgg gacagaaggg cacggtagag
     1321 cctcagctgg aggcccgggg cgacagcaca taccgctgca gctaccagcc caccatggag
     1381 ggcgtccaca ccgtgcacgt cacgtttgcc ggcgtgccca tccctcgcag cccctacact
     1441 gtcactgttg gccaagcctg taacccgagt gcctgccggg cggttggccg gggcctccag
     1501 cccaagggtg tgcgggtgaa ggagacagct gacttcaagg tgtacacaaa gggcgctggc
     1561 agtggggagc tgaaggtcac cgtgaagggc cccaagggag aggagcgcgt gaagcagaag
     1621 gacctggggg atggcgtgta tggcttcgag tattacccca tggtccctgg aacctatatc
     1681 gtcaccatca cgtggggtgg tcagaacatc gggcgcagtc ccttcgaagt gaaggtgggc
     1741 accgagtgtg gcaatcagaa ggtacgggcc tggggccctg ggctggaggg cggcgtcgtt
     1801 ggcaagtcag cagactttgt ggtggaggct atcggggacg acgtgggcac gctgggcttc
     1861 tcggtggaag ggccatcgca ggctaagatc gaatgtgacg acaagggcga cggctcctgt
     1921 gatgtgcgct actggccgca ggaggctggc gagtatgccg ttcacgtgct gtgcaacagc
     1981 gaagacatcc gcctcagccc cttcatggct gacatccgtg acgcgcccca ggacttccac
     2041 ccagacaggg tgaaggcacg tgggcctgga ttggagaaga caggtgtggc cgtcaacaag
     2101 ccagcagagt tcacagtgga tgccaagcac ggtggcaagg ccccacttcg ggtccaagtc
     2161 caggacaatg aaggctgccc tgtggaggcg ttggtcaagg acaacggcaa tggcacttac
     2221 agctgctcct acgtgcccag gaagccggtg aagcacacag ccatggtgtc ctggggaggc
     2281 gtcagcatcc ccaacagccc cttcagggtg aatgtgggag ctggcagcca ccccaacaag
     2341 gtcaaagtat acggccccgg agtagccaag acagggctca aggcccacga gcccacctac
     2401 ttcactgtgg actgcgccga ggctggccag ggggacgtca gcatcggcat caagtgtgcc
     2461 cctggagtgg taggccccgc cgaagctgac atcgacttcg acatcatccg caatgacaat
     2521 gacaccttca cggtcaagta cacgccccgg ggggctggca gctacaccat tatggtcctc
     2581 tttgctgacc aggccacgcc caccagcccc atccgagtca aggtggagcc ctctcatgac
     2641 gccagtaagg tgaaggccga gggccctggc ctcagtcgca ctggtgtcga gcttggcaag
     2701 cccacccact tcacagtaaa tgccaaagct gctggcaaag gcaagctgga cgtccagttc
     2761 tcaggactca ccaaggggga tgcagtgcga gatgtggaca tcatcgacca ccatgacaac
     2821 acctacacag tcaagtacac gcctgtccag cagggtccag taggcgtcaa tgtcacttat
     2881 ggaggggatc ccatccctaa gagccctttc tcagtggcag tatctccaag cctggacctc
     2941 agcaagatca aggtgtctgg cctgggagag aaggtggacg ttggcaaaga ccaggagttc
     3001 acagtcaaat caaagggtgc tggtggtcaa ggcaaagtgg catccaagat tgtgggcccc
     3061 tcgggtgcag cggtgccctg caaggtggag ccaggcctgg gggctgacaa cagtgtggtg
     3121 cgcttcctgc cccgtgagga agggccctat gaggtggagg tgacctatga cggcgtgccc
     3181 gtgcctggca gcccctttcc tctggaagct gtggccccca ccaagcctag caaggtgaag
     3241 gcgtttgggc cggggctgca gggaggcagt gcgggctccc ccgcccgctt caccatcgac
     3301 accaagggcg ccggcacagg tggcctgggc ctgacggtgg agggcccctg tgaggcgcag
     3361 ctcgagtgct tggacaatgg ggatggcaca tgttccgtgt cctacgtgcc caccgagccc
     3421 ggggactaca acatcaacat cctcttcgct gacacccaca tccctggctc cccattcaag
     3481 gcccacgtgg ttccctgctt tgacgcatcc aaagtcaagt gctcaggccc cgggctggag
     3541 cgggccaccg ctggggaggt gggccaattc caagtggact gctcgagcgc gggcagcgcg
     3601 gagctgacca ttgagatctg ctcggaggcg gggcttccgg ccgaggtgta catccaggac
     3661 cacggtgatg gcacgcacac cattacctac attcccctct gccccggggc ctacaccgtc
     3721 accatcaagt acggcggcca gcccgtgccc aacttcccca gcaagctgca ggtggaacct
     3781 gcggtggaca cttccggtgt ccagtgctat gggcctggta ttgagggcca gggtgtcttc
     3841 cgtgaggcca ccactgagtt cagtgtggac gcccgggctc tgacacagac cggagggccg
     3901 cacgtcaagg cccgtgtggc caacccctca ggcaacctga cggagaccta cgttcaggac
     3961 cgtggcgatg gcatgtacaa agtggagtac acgccttacg aggagggact gcactccgtg
     4021 gacgtgacct atgacggcag tcccgtgccc agcagcccct tccaggtgcc cgtgaccgag
     4081 ggctgcgacc cctcccgggt gcgtgtccac gggccaggca tccaaagtgg caccaccaac
     4141 aagcccaaca agttcactgt ggagaccagg ggagctggca cgggcggcct gggcctggct
     4201 gtagagggcc cctccgaggc caagatgtcc tgcatggata acaaggacgg cagctgctcg
     4261 gtcgagtaca tcccttatga ggctggcacc tacagcctca acgtcaccta tggtggccat
     4321 caagtgccag gcagtccttt caaggtccct gtgcatgatg tgacagatgc gtccaaggtc
     4381 aagtgctctg ggcccggcct gagcccaggc atggttcgtg ccaacctccc tcagtccttc
     4441 caggtggaca caagcaaggc tggtgtggcc ccattgcagg tcaaagtgca agggcccaaa
     4501 ggcctggtgg agccagtgga cgtggtagac aacgctgatg gcacccagac cgtcaattat
     4561 gtgcccagcc gagaagggcc ctacagcatc tcagtactgt atggagatga agaggtaccc
     4621 cggagcccct tcaaggtcaa ggtgctgcct actcatgatg ccagcaaggt gaaggccagt
     4681 ggccccgggc tcaacaccac tggcgtgcct gccagcctgc ccgtggagtt caccatcgat
     4741 gcaaaggacg ccggggaggg cctgctggct gtccagatca cggatcccga aggcaagccg
     4801 aagaagacac acatccaaga caaccatgac ggcacgtata cagtggccta cgtgccagac
     4861 gtgacaggtc gctacaccat cctcatcaag tacggtggtg acgagatccc cttctccccg
     4921 taccgcgtgc gtgccgtgcc caccggggac gccagcaagt gcactgtcac aggtgctggc
     4981 atcggcccca ccattcagat tggggaggag acggtgatca ctgtggacac taaggcggca
     5041 ggcaaaggca aagtgacgtg caccgtgtgc acgcctgatg gctcagaggt ggatgtggac
     5101 gtggtggaga atgaggacgg cactttcgac atcttctaca cggcccccca gccgggcaaa
     5161 tacgtcatct gtgtgcgctt tggtggcgag cacgtgccca acagcccctt ccaagtgacg
     5221 gctctggctg gggaccagcc ctcggtgcag ccccctctac ggtctcagca gctggcccca
     5281 cagtacacct acgcccaggg cggccagcag acttgggccc cggagaggcc cctggtgggt
     5341 gtcaatgggc tggatgtgac cagcctgagg ccctttgacc ttgtcatccc cttcaccatc
     5401 aagaagggcg agatcacagg ggaggttcgg atgccctcag gcaaggtggc gcagcccacc
     5461 atcactgaca acaaagacgg caccgtgacc gtgcggtatg cacccagcga ggctggcctg
     5521 cacgagatgg acatccgcta tgacaacatg cacatcccag gaagcccctt gcagttctat
     5581 gtggattacg tcaactgtgg ccatgtcact gcctatgggc ctggcctcac ccatggagta
     5641 gtgaacaagc ctgccacctt caccgtcaac accaaggatg caggagaggg gggcctgtct
     5701 ctggccattg agggcccgtc caaagcagaa atcagctgca ctgacaacca ggatgggaca
     5761 tgcagcgtgt cctacctgcc tgtgctgccg ggggactaca gcattctagt caagtacaat
     5821 gaacagcacg tcccaggcag ccccttcact gcccgggtca caggtgacga ctccatgcgt
     5881 atgtcccacc taaaggtcgg ctctgctgcc gacatcccca tcaacatctc agagacggat
     5941 ctcagcctgc tgacggccac tgtggtcccg ccctcgggcc gggaggagcc ctgtttgctg
     6001 aagcggctgc gtaatggcca cgtggggatt tcattcgtgc ccaaggagac gggggagcac
     6061 ctggtgcatg tgaagaaaaa tggccagcac gtggccagca gccccatccc ggtggtgatc
     6121 agccagtcgg aaattgggga tgccagtcgt gttcgggtct ctggtcaggg ccttcacgaa
     6181 ggccacacct ttgagcctgc agagtttatc attgataccc gcgatgcagg ctatggtggg
     6241 ctcagcctgt ccattgaggg ccccagcaag gtggacatca acacagagga cctggaggac
     6301 gggacgtgca gggtcaccta ctgccccaca gagccaggca actacatcat caacatcaag
     6361 tttgccgacc agcacgtgcc tggcagcccc ttctctgtga aggtgacagg cgagggccgg
     6421 gtgaaagaga gcatcacccg caggcgtcgg gctccttcag tggccaacgt tggtagtcat
     6481 tgtgacctca gcctgaaaat ccctgaaatt agcatccagg atatgacagc ccaggtgacc
     6541 agcccatcgg gcaagaccca tgaggccgag atcgtggaag gggagaacca cacctactgc
     6601 atccgctttg ttcccgctga gatgggcaca cacacagtca gcgtcaagta caagggccag
     6661 cacgtgcctg ggagcccctt ccagttcacc gtggggcccc taggggaagg gggagcccac
     6721 aaggtccgag ctgggggccc tggcctggag agagctgaag ctggagtgcc agccgaattc
     6781 agtatctgga cccgggaagc tggtgctgga ggcctggcca ttgctgtcga gggccccagc
     6841 aaggctgaga tctcttttga ggaccgcaag gacggctcct gtggtgtggc ttatgtggtc
     6901 caggagccag gtgactacga agtctcagtc aagttcaacg aggaacacat tcccgacagc
     6961 cccttcgtgg tgcctgtggc ttctccgtct ggcgacgccc gccgcctcac tgtttctagc
     7021 cttcaggagt cagggctaaa ggtcaaccag ccagcctctt ttgcagtcag cctgaacggg
     7081 gccaaggggg cgatcgatgc caaggtgcac agcccctcag gagccctgga ggagtgctat
     7141 gtcacagaaa ttgaccaaga taagtatgct gtgcgcttca tccctcggga gaatggcgtt
     7201 tacctgattg acgtcaagtt caacggtacc cacatccctg gaagcccctt caagatccga
     7261 gttggggagc ctgggcatgg aggggaccca ggcttggtgt ctgcttacgg agcaggtctg
     7321 gaaggcggtg tcacagggaa cccagctgag ttcgtcgtga acacgagcaa tgcgggagct
     7381 ggtgccctgt cggtgaccat tgacggcccc tccaaggtga agatggattg ccaggagtgc
     7441 cctgagggct accgcgtcac ctataccccc atggcacctg gcagctacct catctccatc
     7501 aagtacggcg gcccctacca cattgggggc agccccttca aggccaaagt cacaggcccc
     7561 cgtctcgtca gcaaccacag cctccacgag acatcatcag tgtttgtaga ctctctgacc
     7621 aaggccacct gtgcccccca gcatggggcc ccgggtcctg ggcctgctga cgccagcaag
     7681 gtggtggcca agggcctggg gctgagcaag gcctacgtag gccagaagag cagcttcaca
     7741 gtagactgca gcaaagcagg caacaacatg ctgctggtgg gggttcatgg cccaaggacc
     7801 ccctgcgagg agatcctggt gaagcacgtg ggcagccggc tctacagcgt gtcctacctg
     7861 ctcaaggaca agggggagta cacactggtg gtcaaatggg ggcacgagca catcccaggc
     7921 agcccctacc gcgttgtggt gcccagcggc agcgcctcac cggtcgccac catggtgagc
     7981 aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg
     8041 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
     8101 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg
     8161 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac
     8221 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
     8281 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc
     8341 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag
     8401 aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag
     8461 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag
     8521 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag
     8581 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag
     8641 ggccgccact ccaccggcgg catggacgag ctgtacaagt aaagcggccg cgactctaga
     8701 tcataatcag ccataccaca t
//
