LOCUS       sequence-85433-e        2003 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin , Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2003)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Gamma-Actin2-C-18
FEATURES             Location/Qualifiers
     source          1..2003
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      24..33
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             30..737
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(175..193)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     443..462
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    1920..1972
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 cagatccgct agcgctaccg gtcgccacca tggtgagcaa gggcgaggag gataacatgg
       61 ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg
      121 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
      181 tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca
      241 tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt
      301 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga
      361 ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg
      421 gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
      481 cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga
      541 agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc
      601 ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg
      661 aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca
      721 tggacgagct gtacaagtcc ggagggtcca gcggcggtgg atctgggtcc agcggaagta
      781 gcggcagcgg ctgtgaagaa gagaccaccg cccttgtgtg tgacaatggc tctggcctgt
      841 gcaaggcagg ctttgcagga gatgatgccc ccagggctgt cttcccctcc attgtgggcc
      901 gccctagaca tcagggtgtg atggtgggaa tgggccagaa agacagctat gtgggggacg
      961 aggctcagag caagcgtggg atcctaactc tcaagtaccc tattgaacat ggcatcatca
     1021 ccaactggga tgacatggag aagatctggc accactcctt ctacaatgag cttcgagtag
     1081 caccagaaga gcaccccacc ctgctcacag aggcccccct aaaccccaaa gcaaacaggg
     1141 agaagatgac ccagatcatg ttcgaaacct tcaatgtccc tgccatgtat gttgctattc
     1201 aggctgtgct ctcactctat gcatccggcc gtaccacagg catcgtcctg gattcggggg
     1261 atggcgtcac ccacaatgtc cccatctatg agggctatgc actgccccat gccatcatgc
     1321 gtcttgacct ggctggacgg gatctcacag actacctcat gaagattctc acagaaagag
     1381 gctattcctt tgtgaccaca gctgagagag aaattgtacg agacatcaag gagaagctgt
     1441 gctatgtagc cctggatttc gagaatgaga tggccacagc agcttcatct tcttccctgg
     1501 agaaaagcta cgagttgcct gatgggcagg tcatcactat tggcaacgaa cgcttccgct
     1561 gcccggagac cctcttccag ccttccttca ttggcatgga gtcagctgga attcatgaaa
     1621 caacatacaa ttccatcatg aagtgtgaca ttgacatccg caaagatttg tatgctaaca
     1681 atgtcctctc tgggggcact accatgtacc ctggcattgc tgacaggatg cagaaggaaa
     1741 tcacagcctt ggctcccagc accatgaaga tcaagattat cgctcctcct gagcggaagt
     1801 actcagtctg gattggcggc tccatcctgg cctctctctc caccttccag caaatgtgga
     1861 tcagcaagcc agagtatgat gaggcagggc cctccattgt ccacaggaaa tgcttctaac
     1921 tcgagctcaa gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat ccaccggatc
     1981 tagataactg atcataatca gcc
//
