LOCUS       sequence-85434-e        2176 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Centrosomes, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2176)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Gamma-Tubulin-17
FEATURES             Location/Qualifiers
     source          1..2176
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1421..1430
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1427..2137
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1572..1590)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1840..1859
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cgtcagatcc gctagcgcca ccatgccgag ggaaatcatc accctacagt tgggccagtg
       61 cggcaatcag attgggttcg agttctggaa acagctgtgc gccgagcatg gtatcagccc
      121 cgagggcatc gtggaggagt tcgccaccga gggcactgac cacaaggacg tctttttcta
      181 ccaggcagac gatgagcact acatcccccg ggccgtgctg ctggacttgg aaccccgggt
      241 gatccactcc atcctcaact ccccctatgc caagctctac aacccagaga acatctacct
      301 gtcggaacat ggaggaggag ctggcaacaa ctgggccagc ggattctccc agggagaaaa
      361 gatccatgag gacatttttg acatcataga ccgggaggca gatggtagtg acagtctaga
      421 gggctttgtg ctgtgtcact ccattgctgg ggggacaggc tctggactgg gttcctacct
      481 cttagaacgg ctgaatgaca ggtatcctaa gaagctggtg cagacatact cagtgtttcc
      541 caaccaggac gagatgagcg atgtggtggt ccagccttac aattcactcc tcacactcaa
      601 gaggctgacg cagaatgcag actgtgtggt ggtgctggac aacacagccc tgaaccggat
      661 tgccacagac cgcctgcaca tccagaaccc atccttctcc cagatcaacc agctggtgtc
      721 taccatcatg tcagccagca ccaccaccct gcgctaccct ggctacatga acaatgacct
      781 catcggcctc atcgcctcgc tcattcccac cccacggctc cacttcctca tgaccggcta
      841 cacccctctc actacggacc agtcagtggc cagcgtgagg aagaccacgg tcctggatgt
      901 catgaggcgg ctgctgcagc ccaagaacgt gatggtgtcc acaggccgag accgccagac
      961 caaccactgc tacatcgcca tcctcaacat catccaggga gaggtggacc ccacccaggt
     1021 ccacaagagc ttgcagagga tccgggaacg caagttggcc aacttcatcc cgtggggccc
     1081 cgccagcatc caggtggccc tgtcgaggaa gtctccctac ctgccctcgg cccaccgggt
     1141 cagcgggctc atgatggcca accacaccag catctcctcg ctcttcgaga gaacctgtcg
     1201 ccagtatgac aagctgcgta agcgggaggc cttcctggag cagttccgca aggaggacat
     1261 gttcaaggac aactttgatg agatggacac atccagggag attgtgcagc agctcatcga
     1321 tgagtaccat gcggccacac ggccagacta catctcctgg ggcactcagg agcagcgaat
     1381 tctgcagtcg acggtaccgc gggcccggga tccaccggtc gccaccatgg tgagcaaggg
     1441 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
     1501 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     1561 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
     1621 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
     1681 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     1741 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
     1801 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
     1861 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
     1921 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
     1981 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga
     2041 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
     2101 ccactccacc ggcggcatgg acgagctgta caagtaaagc ggccgcgact ctagatcata
     2161 atcagccata ccacat
//
