LOCUS       sequence-85435-e        2121 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Intermediate Filaments, Excitation: 587, Emission: 
            610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2121)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-GFAP-N-18
FEATURES             Location/Qualifiers
     source          1..2121
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      16..25
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1366..1375
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1372..2082
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1517..1535)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1785..1804
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gtcagatccg ctagcgccac catggagagg agacgcatca cctccgctgc tcgccgctcc
       61 tacgtctcct caggggagat gatggtgggg ggcctggctc ctggccgccg tctgggtcct
      121 ggcacccgcc tctccctggc tcgaatgccc cctccactcc caacccgggt ggatttctcc
      181 ctggctgggg cactcaatgc tggcttcaag gagacccggg ccagtgagcg ggcagagatg
      241 atggagctca atgaccgctt tgccagctac atcgagaagg ttcgcttcct ggaacagcaa
      301 aacaaggcgc tggctgctga gctgaaccag ctgcgggcca aggagcccac caagctggca
      361 gacgtctacc aggctgagct gcgagagctg cggctgcggc tcgatcaact caccgccaac
      421 agcgcccggc tggaggttga gagggacaat ctggcacagg acctggccac tgtgaggcag
      481 aagctccagg atgaaaccaa cctgaggctg gaagccgaga acaacctggc tgcctataga
      541 caggaagcag atgaagccac cctggcccgt ctggatctgg agaggaagat tgagtcgctg
      601 gaggaggaga tccggttctt gaggaagatc cacgaggagg aggttcggga actccaggag
      661 cagctggccc gacagcaggt ccatgtggag cttgacgtgg ccaagccaga cctcaccgca
      721 gccctgaaag agatccgcac gcagtatgag gcaatggcgt ccagcaacat gcatgaagcc
      781 gaagagtggt accgctccaa gtttgcagac ctgacagacg ctgctgcccg caacgcggag
      841 ctgctccgcc aggccaagca cgaagccaac gactaccggc gccagttgca gtccttgacc
      901 tgcgacctgg agtctctgcg cggcacgaac gagtccctgg agaggcagat gcgcgagcag
      961 gaggagcggc acgtgcggga ggcggccagt tatcaggagg cgctggcgcg gctggaggaa
     1021 gaggggcaga gcctcaagga cgagatggcc cgccacttgc aggagtacca ggacctgctc
     1081 aatgtcaagc tggccctgga catcgagatc gccacctaca ggaagctgct agagggcgag
     1141 gagaaccgga tcaccattcc cgtgcagacc ttctccaacc tgcagattcg agaaaccagc
     1201 ctggacacca agtctgtgtc agaaggccac ctcaagagga acatcgtggt gaagaccgtg
     1261 gagatgcggg atggagaggt cattaaggag tccaagcagg agcacaagga tgtgatgagc
     1321 agcggtggag caagcgcagc cagtggtagc gcggatccac cggtcgccac catggtgagc
     1381 aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg
     1441 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
     1501 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg
     1561 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac
     1621 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
     1681 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc
     1741 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag
     1801 aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag
     1861 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag
     1921 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag
     1981 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag
     2041 ggccgccact ccaccggcgg catggacgag ctgtacaagt aaagcggccg cgactctaga
     2101 tcataatcag ccataccaca t
//
