LOCUS       sequence-85436-e        1034 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Golgi Complex, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1034)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Golgi-7
FEATURES             Location/Qualifiers
     source          1..1034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      278..287
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             284..994
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(429..447)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     697..716
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cgtcagatcc gctagcatga ggcttcggga gccgctcctg agcggcagcg ccgcgatgcc
       61 aggcgcgtcc ctacagcggg cctgccgcct gctcgtggcc gtctgcgctc tgcaccttgg
      121 cgtcaccctc gtttactacc tggctggccg cgacctgagc cgcctgcccc aactggtcgg
      181 agtctccaca ccgctgcagg gcggctcgaa cagtgccgcc gccatcgggc agtcctccgg
      241 ggagctccgg accggagggg ccaaggatcc accggtcgcc accatggtga gcaagggcga
      301 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
      361 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
      421 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
      481 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
      541 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
      601 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
      661 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
      721 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
      781 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
      841 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
      901 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
      961 ctccaccggc ggcatggacg agctgtacaa gtaaagcggc cgcgactcta gatcataatc
     1021 agccatacca catt
//
