LOCUS       sequence-85437-e        1388 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1388)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-H1-10
FEATURES             Location/Qualifiers
     source          1..1388
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      632..641
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             638..1348
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(783..801)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1051..1070
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gacgtcagat ccgctagccg ccaccatgac cgagaactcc acctccgccc cggcggcgaa
       61 gcccaaacgg gccaaggctt ccaagaagtc cacggaccac cccaagtatt cagacatgat
      121 cgtggctgct atccaggcag agaagaaccg tgccggctcc tcgcgccagt ccatccaaaa
      181 gtatatcaag agccactaca aggtgggtga gaacgccgac tcccagatca agttgtccat
      241 caagcgccta gtgaccaccg gtgttctcaa gcaaaccaaa ggggtgggcg cctcggggtc
      301 cttcaggctg gccaagggcg atgagcccaa aaggtcggtg gctttcaaga agaccaagaa
      361 ggaagtcaag aaagtggcca ctccaaagaa ggcagccaag cccaagaagg ctgcctccaa
      421 agccccaagc aagaaaccca aagccacccc tgtcaagaag gccaagaaga agccggctgc
      481 cacgcccaag aaagccaaaa agcccaaggt tgtcaaagtc aaaccagtca aggcctccaa
      541 acccaagaag gccaaaaccg tgaagcccaa agccaagtcg agtgccaaga gggggagcaa
      601 gaagaagtcg ggaagcacgg atccaccggt cgccaccatg gtgagcaagg gcgaggagga
      661 taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa
      721 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
      781 cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc
      841 tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt
      901 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
      961 cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa
     1021 gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg
     1081 ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca
     1141 gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc
     1201 caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc
     1261 ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac
     1321 cggcggcatg gacgagctgt acaagtaaag cggccgcgac tctagatcat aatcagccat
     1381 accacatt
//
