LOCUS       sequence-85439-e        1263 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1263)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1263)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-H2B-18
FEATURES             Location/Qualifiers
     source          1..1263
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             80..457
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      506..515
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             512..1222
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(657..675)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     925..944
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tgacgtcaga tccgctagcg ctaccggact cagatctcga gctcaagctt cgaattctgc
       61 agtcgacggt accgccacca tgccagagcc agcgaagtct gctcccgccc cgaaaaaggg
      121 ctccaagaag gcggtgacta aggcgcagaa gaaaggcggc aagaagcgca agcgcagccg
      181 caaggagagc tattccatct atgtgtacaa ggttctgaag caggtccacc ctgacaccgg
      241 catttcgtcc aaggccatgg gcatcatgaa ttcgtttgtg aacgacattt tcgagcgcat
      301 cgcaggtgag gcttcccgcc tggcgcatta caacaagcgc tcgaccatca cctccaggga
      361 gatccagacg gccgtgcgcc tgctgctgcc tggggagttg gccaagcacg ccgtgtccga
      421 gggtactaag gccatcacca agtacaccag cgctaaggat cacggtcacg gtactggttc
      481 tactggttct ggttcttctc cggtcgccac catggtgagc aagggcgagg aggataacat
      541 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca
      601 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
      661 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt
      721 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct
      781 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
      841 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg
      901 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
      961 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct
     1021 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa
     1081 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa
     1141 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg
     1201 catggacgag ctgtacaagt aaagcggccg cgactctaga tcataatcag ccataccaca
     1261 ttt
//
