LOCUS       sequence-8544-ex        3544 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Merritt C, Rasoloson D, Ko D, Seydoux G
  TITLE     3' UTRs are the primary regulators of gene expression in the C. 
            elegans germline.
  JOURNAL   Curr Biol. 2008 Oct 14;18(19):1476-82. doi: 
            10.1016/j.cub.2008.08.013. Epub 2008 Sep 25.
  PUBMED    18818082
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2008.08.013"; journalName: "Curr Biol"; date: 
            "2008-10-14- 14"; volume: "18"; issue: "19"; pages: "1476-82"
COMMENT     Sequence Label: pCM1.35
FEATURES             Location/Qualifiers
     source          1..3544
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(73..100)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(192..278)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    331..430
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(463..491)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1239..1259
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    complement(1720..1819)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1931..1950)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1942..2751
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2009..2028)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2894..3482
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3383..3402
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagcc aggaagagtt
       61 tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca
      121 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc
      181 gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
      241 aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct
      301 cgcgttaacg ctagcatgga tctcgggccc caaataatga ttttattttg actgatagtg
      361 acctgttcgt tgcaacaaat tgatgagcaa tgctttttta taatgccaag tttgtacaaa
      421 aaagcaggct ccatgagtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt
      481 gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat
      541 gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca
      601 tgggtaagtt taaacatata tatactaact aaccctgatt atttaaattt tcagccaaca
      661 cttgtcacta ctttctgtta tggtgttcaa tgcttctcga gatacccaga tcatatgaaa
      721 cggcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt
      781 ttcaaagatg acgggaacta caagacacgt aagtttaaac agttcggtac taactaacca
      841 tacatattta aattttcagg tgctgaagtc aagtttgaag gtgataccct tgttaataga
      901 atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa
      961 tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa
     1021 gttgtaagtt taaacatgat tttactaact aactaatctg atttaaattt tcagaacttc
     1081 aaaattagac acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat
     1141 actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct
     1201 gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca
     1261 gctgctggga ttacacatgg catggatgaa ctatacaaat gcccggggga tcggtggagc
     1321 tccaccggtg gcggccgctc tagaactagt ccaccaaagc catctgccaa gggagccaag
     1381 aaggccgcca agaccgtcgt tgccaagcca aaggacggaa agaagagacg tcatgcccgc
     1441 aaggaatcgt actccgtcta catctaccgt gttctcaagc aagttcaccc agacaccgga
     1501 gtctcctcca aggccatgtc tatcatgaac tccttcgtca acgatgtatt cgaacgcatc
     1561 gcttcggaag cttcccgtct tgctcattac aacaaacgct caacgatctc atcccgcgaa
     1621 attcaaaccg ctgtccgttt gattctccca ggagaacttg ccaagcacgc cgtgtctgag
     1681 ggaaccaagg ccgtcaccaa gtacacttcc agcaagtaaa cccagctttc ttgtacaaag
     1741 tgggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc
     1801 aaaataaaat cattatttgc catccagctg cagctctggc ccgtgtctca aaatctctga
     1861 tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata
     1921 aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta
     1981 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
     2041 tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa
     2101 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg
     2161 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
     2221 ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat
     2281 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct
     2341 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga
     2401 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
     2461 gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact
     2521 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt
     2581 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc
     2641 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
     2701 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg
     2761 gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca
     2821 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     2881 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     2941 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     3001 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     3061 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     3121 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     3181 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     3241 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     3301 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     3361 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     3421 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     3481 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     3541 tgtt
//
