LOCUS       sequence-85440-e        1162 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Kuipers MA, Stasevich TJ, Sasaki T, Wilson KA, Hazelwood KL, McNally
            JG, Davidson MW, Gilbert DM
  TITLE     Highly stable loading of Mcm proteins onto chromatin in living cells
            requires replication to unload.
  JOURNAL   J Cell Biol. 2011 Jan 10;192(1):29-41. doi: 10.1083/jcb.201007111.
  PUBMED    21220507
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2011-01-10- 10"; volume: "192"; 
            issue: "1"; pages: "29-41"
COMMENT     Sequence Label: mCherry-H2B-6
FEATURES             Location/Qualifiers
     source          1..1162
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..390
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      403..412
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             409..1119
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(554..572)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     822..841
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ggtaccgcca ccatgccaga gccagcgaag tctgctcccg ccccgaaaaa gggctccaag
       61 aaggcggtga ctaaggcgca gaagaaaggc ggcaagaagc gcaagcgcag ccgcaaggag
      121 agctattcca tctatgtgta caaggttctg aagcaggtcc accctgacac cggcatttcg
      181 tccaaggcca tgggcatcat gaattcgttt gtgaacgaca ttttcgagcg catcgcaggt
      241 gaggcttccc gcctggcgca ttacaacaag cgctcgacca tcacctccag ggagatccag
      301 acggccgtgc gcctgctgct gcctggggag ttggccaagc acgccgtgtc cgagggtact
      361 aaggccatca ccaagtacac cagcgctaag gatccaccgg tcgccaccat ggtgagcaag
      421 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
      481 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
      541 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
      601 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
      661 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
      721 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
      781 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
      841 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
      901 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
      961 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg
     1021 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
     1081 cgccactcca ccggcggcat ggacgagctg tacaagtaaa gcggccgcga ctctagatca
     1141 taatcagcca taccacattg ag
//
