LOCUS       sequence-85442-e        1253 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1253)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1253)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-H3-23
FEATURES             Location/Qualifiers
     source          1..1253
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    437..489
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      497..506
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             503..1213
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(648..666)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     916..935
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gacgtcagat ccgctagccg ccaccatggc tcgtactaaa cagacagctc ggaaatccac
       61 cggcggtaaa gcgccacgca agcagctggc taccaaggct gctcgcaaga gcgcgccggc
      121 taccggcggc gtgaaaaagc ctcaccgtta ccgcccgggc actgtggctc tgcgcgagat
      181 ccgccgctac caaaagtcga ccgagttgct gattcggaag ctgccgttcc agcgcctggt
      241 gcgagaaatc gcccaagact tcaagaccga tcttcgcttc cagagctctg cggtgatggc
      301 gctgcaggag gcttgtgagg cctacttggt agggctcttt gaggacacaa acctttgcgc
      361 catccatgct aagcgagtga ctattatgcc caaagacatc cagctcgctc gccgcattcg
      421 cggagaaaga gcggatctcg agctcaagct tcgaattctg cagtcgacgg taccgcgggc
      481 ccgggatcca ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat
      541 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
      601 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
      661 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg
      721 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
      781 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
      841 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
      901 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga
      961 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa
     1021 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca
     1081 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta
     1141 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga
     1201 gctgtacaag taaagcggcc gcgactctag atcataatca gccataccac att
//
