LOCUS       sequence-85443-e        1246 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1246)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-H3.3-N-14
FEATURES             Location/Qualifiers
     source          1..1246
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      39..48
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      489..498
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             495..1205
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(640..658)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     908..927
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gctagcgcta ccggactcag atctcgagct caagcttcgc caccatggcc cgaaccaagc
       61 agaccgctag gaagtccacc ggtgggaaag ccccccgcaa acagctggcc accaaggcgg
      121 ctcggaaaag cgcgccctct accggcgggg tgaagaagcc tcaccgctac aggccaggga
      181 ccgtggctct gagagagatc cgtcgttacc agaaatcgac tgagctgctc atccggaagc
      241 tgccattcca gagattggtg agggagatcg cccaggattt caaaaccgac ttgaggtttc
      301 aaagtgcagc catcggtgcc cttcaggagg ctagcgaagc atacctggtg gggttgtttg
      361 aagataccaa tctgtgtgcc atccacgcca agagagtcac catcatgccc aaagacatcc
      421 agttggctcg ccggatacgg ggggagagag ctgggtccgg aagcgcaggc agtggggatc
      481 caccggtcgc caccatggtg agcaagggcg aggaggataa catggccatc atcaaggagt
      541 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg
      601 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
      661 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg
      721 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct
      781 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact
      841 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
      901 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt
      961 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg
     1021 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg
     1081 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg
     1141 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca
     1201 agtaaagcgg ccgcgactct agatcataat cagccatacc acattt
//
