LOCUS       sequence-85446-e        1231 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1231)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1231)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Hec1-N-12
FEATURES             Location/Qualifiers
     source          1..1231
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      463..472
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             469..1179
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(614..632)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     882..901
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ttggaattgg ccattgtata ccgacggcag gaggttggga cctgagagag tcaagctgat
       61 gatcctacac aaaagtaagg aagcagagag agatgaaaat gtgcagtgag ctgagtctga
      121 gaaacacagc acctgctaga aagtactgta gcagggctca gtgagctatg aatgaattag
      181 atgctgtcag cgggaatacc aactagttgt gcaaacacga ctgaagaaag acgaaaagtg
      241 ggaaataact tgcaacgtct gttagagatg gttgctacac atgttgggtc tgtagagaaa
      301 catcttgagg agcagattgc taaagttgat agagaatatg aagaatgcat gtcagaagat
      361 ctctcggaaa atattaaaga gattagagat aagtatgaga agaaagctac tctaattaag
      421 tcttctgaag aacggtaccg cgggcccggg atccaccggg tcgccaccat ggtgagcaag
      481 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
      541 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
      601 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
      661 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
      721 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
      781 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
      841 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
      901 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
      961 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
     1021 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg
     1081 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
     1141 cgccactcca ccggcggcat ggacgagctg tacaagtaaa gcggccgcga ctctagatca
     1201 taatcagcca taccacattg tagagtatcc t
//
