LOCUS       sequence-85447-e        2201 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2201)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-ILK-N-17
FEATURES             Location/Qualifiers
     source          1..2201
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1449..1458
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1455..2165
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1600..1618)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1868..1887
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tgacgtcaga tccgctagcg ctaccggact cagatctcga gaccgccatg gacgacattt
       61 tcactcagtg ccgggagggc aacgcagtcg ccgttcgcct gtggctggac aacacggaga
      121 acgacctcaa ccagggggac gatcatggct tctccccctt gcactgggcc tgccgagagg
      181 gccgctctgc tgtggttgag atgttgatca tgcggggggc acggatcaat gtaatgaacc
      241 gtggggatga cacccccctg catctggcag ccagtcatgg acaccgtgat attgtacaga
      301 agctattgca gtacaaggca gacatcaatg cagtgaatga acatgggaat gtgcccctgc
      361 actatgcctg tttttggggc caagatcaag tggcagagga cctggtggca aatggggccc
      421 ttgtcagcat ctgtaacaag tatggagaga tgcctgtgga caaagccaag gcacccctga
      481 gagagcttct ccgagagcgg gcagagaaga tgggccagaa tctcaaccgt attccataca
      541 aggacacatt ctggaagggg accacccgca ctcggccccg aaatggaacc ctgaacaaac
      601 actctggcat tgacttcaaa cagcttaact tcctgacgaa gctcaacgag aatcactctg
      661 gagagctatg gaagggccgc tggcagggca atgacattgt cgtgaaggtg ctgaaggttc
      721 gagactggag tacaaggaag agcagggact tcaatgaaga gtgtccccgg ctcaggattt
      781 tctcgcatcc aaatgtgctc ccagtgctag gtgcctgcca gtctccacct gctcctcatc
      841 ctactctcat cacacactgg atgccatatg gatccctcta caatgtacta catgaaggca
      901 ccaatttcgt cgtggaccag agccaggctg tgaagtttgc tttggacatg gcaaggggca
      961 tggccttcct acacacacta gagcccctca tcccacgaca tgcactcaat agccgtagtg
     1021 taatgattga tgaggacatg actgcccgaa ttagcatggc tgatgtcaag ttctctttcc
     1081 aatgtcctgg tcgcatgtat gcacctgcct gggtagcccc cgaagctctg cagaagaagc
     1141 ctgaagacac aaacagacgc tcagcagaca tgtggagttt tgcagtgctt ctgtgggaac
     1201 tggtgacacg ggaggtaccc tttgctgacc tctccaatat ggagattgga atgaaggtgg
     1261 cattggaagg ccttcggcct accatcccac caggtatttc ccctcatgtg tgtaagctca
     1321 tgaagatctg catgaatgaa gaccctgcaa agcgacccaa atttgacatg attgtgccta
     1381 tccttgagaa gatgcaggac aagggaattc tgcagtcgac ggtaccgcgg gcccgggatc
     1441 caccggtcgc caccatggtg agcaagggcg aggaggataa catggccatc atcaaggagt
     1501 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg
     1561 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
     1621 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg
     1681 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct
     1741 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact
     1801 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
     1861 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt
     1921 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg
     1981 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg
     2041 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg
     2101 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca
     2161 agtaaagcgg ccgcgactct agatcataat cagccatacc a
//
