LOCUS       sequence-85450-e        2229 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytokeratin, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2229)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Keratin14-N-18
FEATURES             Location/Qualifiers
     source          1..2229
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1485..1494
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1491..2201
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1636..1654)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1904..1923
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tcagatccgc tagcgccacc atgactacct gcagccgcca gttcacctcc tccagctcca
       61 tgaagggctc ctgcggcatc gggggcggca tcgggggcgg ctccagccgc atctcctccg
      121 tcctggccgg agggtcctgc cgcgccccca gcacctacgg gggcggcctg tctgtctcat
      181 cctcccgctt ctcctctggg ggagcctatg ggttgggggg cggctatggc ggtggcttca
      241 gcagcagcag cagcagcttt ggtagtggct ttgggggagg atatggtggt ggccttggtg
      301 ctggcttggg tggtggcttt ggtggtggct ttgctggtgg tgatgggctt ctggtgggca
      361 gtgagaaggt gaccatgcag aacctcaacg accgcctggc ctcctacctg gacaaggtgc
      421 gtgctctgga ggaggccaac gccgacctgg aagtgaagat ccgtgactgg taccagaggc
      481 agcggcctgc tgagatcaaa gactacagtc cctacttcaa gaccattgag gacctgagga
      541 acaagattct cacagccaca gtggacaatg ccaatgtcct tctgcagatt gacaatgccc
      601 gtctggccgc ggatgacttc cgcaccaagt atgagacaga gttgaacctg cgcatgagtg
      661 tggaagccga catcaatggc ctgcgcaggg tgctggacga actgaccctg gccagagctg
      721 acctggagat gcagattgag agcctgaagg aggagctggc ctacctgaag aagaaccacg
      781 aggaggagat gaatgccctg agaggccagg tgggtggaga tgtcaatgtg gagatggacg
      841 ctgcacctgg cgtggacctg agccgcattc tgaacgagat gcgtgaccag tatgagaaga
      901 tggcagagaa gaaccgcaag gatgccgagg aatggttctt caccaagaca gaggagctga
      961 accgcgaggt ggccaccaac agcgagctgg tgcagagcgg caagagcgag atctcggagc
     1021 tccggcgcac catgcagaac ctggagattg agctgcagtc ccagctcagc atgaaagcat
     1081 ccctggagaa cagcctggag gagaccaaag gtcgctactg catgcagctg gcccagatcc
     1141 aggagatgat tggcagcgtg gaggagcagc tggcccagct ccgctgcgag atggagcagc
     1201 agaaccagga gtacaagatc ctgctggacg tgaagacgcg gctggagcag gagatcgcca
     1261 cctaccgccg cctgctggag ggcgaggacg cccacctctc ctcctcccag ttctcctctg
     1321 gatcgcagtc atccagagat gtgacctcct ccagccgcca aatccgcacc aaggtcatgg
     1381 atgtgcacga tggcaaggtg gtgtccaccc acgagcaggt ccttcgcacc aagaacagca
     1441 gcggtggagc aagcgcagcc agtggtagcg cggatccacc ggtcgccacc atggtgagca
     1501 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
     1561 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     1621 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
     1681 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
     1741 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     1801 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
     1861 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
     1921 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     1981 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     2041 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     2101 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     2161 gccgccactc caccggcggc atggacgagc tgtacaagta aagcggccgc gactctagat
     2221 cataatcag
//
