LOCUS       sequence-85452-e        2819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear Envelope, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2819)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-LaminA-C-18
FEATURES             Location/Qualifiers
     source          1..2819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             33..740
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(178..196)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     446..465
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cgtcagatcc gctagcgcta ccggtcgcca ccatggtgag caagggcgag gaggataaca
       61 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc
      121 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
      181 agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt
      241 tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc
      301 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg
      361 tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc
      421 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg
      481 cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc
      541 tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga
      601 agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca
      661 acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg
      721 gcatggacga gctgtacaag tccggactca gatctggcag cggtggaggc agcgcatccg
      781 gcggaagcgg aagcgagacc ccgtcccagc ggcgcgccac ccgcagcggg gcgcaggcca
      841 gctccactcc gctgtcgccc acccgcatca cccggctgca ggagaaggag gacctgcagg
      901 agctcaatga tcgcttggcg gtctacatcg accgtgtgcg ctcgctggaa acggagaacg
      961 cagggctgcg ccttcgcatc accgagtctg aagaggtggt cagccgcgag gtgtccggca
     1021 tcaaggccgc ctacgaggcc gagctcgggg atgcccgcaa gacccttgac tcagtagcca
     1081 aggagcgcgc ccgcctgcag ctggagctga gcaaagtgcg tgaggagttt aaggagctga
     1141 aagcgcgcaa taccaagaag gagggtgacc tgatagctgc tcaggctcgg ctgaaggacc
     1201 tggaggctct gctgaactcc aaggaggccg cactgagcac tgctctcagt gagaagcgca
     1261 cgctggaggg cgagctgcat gatctgcggg gccaggtggc caagcttgag gcagccctag
     1321 gtgaggccaa gaagcaactt caggatgaga tgctgcggcg ggtggatgct gagaacaggc
     1381 tgcagaccat gaaggaggaa ctggacttcc agaagaacat ctacagtgag gagctgcgtg
     1441 agaccaagcg ccgtcatgag acccgactgg tggagattga caatgggaag cagcgtgagt
     1501 ttgagagccg gctggcggat gcgctgcagg aactgcgggc ccagcatgag gaccaggtgg
     1561 agcagtataa gaaggagctg gagaagactt attctgccaa gctggacaat gccaggcagt
     1621 ctgctgagag gaacagcaac ctggtggggg ctgcccacga ggagctgcag cagtcgcgca
     1681 tccgcatcga cagcctctct gcccagctca gccagctcca gaagcagctg gcagccaagg
     1741 aggcgaagct tcgagacctg gaggactcac tggcccgtga gcgggacacc agccggcggc
     1801 tgctggcgga aaaggagcgg gagatggccg agatgcgggc aaggatgcag cagcagctgg
     1861 acgagtacca ggagcttctg gacatcaagc tggccctgga catggagatc cacgcctacc
     1921 gcaagctctt ggagggcgag gaggagaggc tacgcctgtc ccccagccct acctcgcagc
     1981 gcagccgtgg ccgtgcttcc tctcactcat cccagacaca gggtgggggc agcgtcacca
     2041 aaaagcgcaa actggagtcc actgagagcc gcagcagctt ctcacagcac gcacgcacta
     2101 gcgggcgcgt ggccgtggag gaggtggatg aggagggcaa gtttgtccgg ctgcgcaaca
     2161 agtccaatga ggaccagtcc atgggcaatt ggcagatcaa gcgccagaat ggagatgatc
     2221 ccttgctgac ttaccggttc ccaccaaagt tcaccctgaa ggctgggcag gtggtgacga
     2281 tctgggctgc aggagctggg gccacccaca gcccccctac cgacctggtg tggaaggcac
     2341 agaacacctg gggctgcggg aacagcctgc gtacggctct catcaactcc actggggaag
     2401 aagtggccat gcgcaagctg gtgcgctcag tgactgtggt tgaggacgac gaggatgagg
     2461 atggagatga cctgctccat caccaccacg gctcccactg cagcagctcg ggggaccccg
     2521 ctgagtacaa cctgcgctcg cgcaccgtgc tgtgcgggac ctgcgggcag cctgccgaca
     2581 aggcatctgc cagcggctca ggagcccagg tgggcggacc catctcctct ggctcttctg
     2641 cctccagtgt cacggtcact cgcagctacc gcagtgtggg gggcagtggg ggtggcagct
     2701 tcggggacaa tctggtcacc cgctcctacc tcctgggcaa ctccagcccc cgaacccaga
     2761 gcccccagaa ctgcagcatc atgtaaggat ccaccggatc tagataactg atcataatc
//
