LOCUS       sequence-85454-e        1603 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1603)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Lasp1-C-10
FEATURES             Location/Qualifiers
     source          1..1603
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      30..39
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             36..743
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(181..199)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     449..468
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     regulatory      958..967
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tgacgtcaga tccgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggaggata
       61 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
      121 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
      181 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
      241 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
      301 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
      361 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
      421 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
      481 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
      541 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
      601 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
      661 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
      721 gcggcatgga cgagctgtac aagtccggac tcagatctag cggtggcagt ggaaacccca
      781 actgcgcccg gtgcggcaag atcgtgtatc ccacggagaa ggtgaactgt ctggataagt
      841 tctggcataa agcatgcttc cattgcgaga cctgcaagat gacactgaac atgaagaact
      901 acaagggcta cgagaagaag ccctactgca acgcacacta ccccaagcag tccttcacca
      961 tggtggcgga caccccggaa aaccttcgcc tcaagcaaca gagtgagctc cagagtcagg
     1021 tgcgctacaa ggaggagttt gagaagaaca agggcaaagg tttcagcgta gtggcagaca
     1081 cgcccgagct ccagagaatc aagaagaccc aggaccagat cagtaacata aaataccatg
     1141 aggagtttga gaagagccgc atgggcccta gcgggggcga gggcatggag ccagagcgtc
     1201 gggattcaca ggacggcagc agctaccggc ggcccctgga gcagcagcag cctcaccaca
     1261 tcccgaccag tgccccggtt taccagcagc cccagcagca gccggtggcc cagtcctatg
     1321 gtggctacaa ggagcctgca gccccagtct ccatacagcg cagcgcccca ggtggtggcg
     1381 ggaagcggta ccgcgtggtg tatgactaca gcgccgccga cgaggacgag gtctccttcc
     1441 aggacgggga caccatcgtc aacgtgcagc agatcgacga cggctggatg tacgggacgg
     1501 tggagcgcac cggcgacacg gggatgctgc cggccaacta cgtggaggcc atctgaggat
     1561 ccaccggatc tagataactg atcataatca gccataccac att
//
