LOCUS       sequence-85455-e        1582 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1582)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Lasp1-N-10
FEATURES             Location/Qualifiers
     source          1..1582
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      206..215
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      826..835
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             832..1542
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(977..995)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1245..1264
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 agatccgcta gcgccaccat gaaccccaac tgcgcccggt gcggcaagat cgtgtatccc
       61 acggagaagg tgaactgtct ggataagttc tggcataaag catgcttcca ttgcgagacc
      121 tgcaagatga cactgaacat gaagaactac aagggctacg agaagaagcc ctactgcaac
      181 gcacactacc ccaagcagtc cttcaccatg gtggcggaca ccccggaaaa ccttcgcctc
      241 aagcaacaga gtgagctcca gagtcaggtg cgctacaagg aggagtttga gaagaacaag
      301 ggcaaaggtt tcagcgtagt ggcagacacg cccgagctcc agagaatcaa gaagacccag
      361 gaccagatca gtaacataaa ataccatgag gagtttgaga agagccgcat gggccctagc
      421 gggggcgagg gcatggagcc agagcgtcgg gattcacagg acggcagcag ctaccggcgg
      481 cccctggagc agcagcagcc tcaccacatc ccgaccagtg ccccggttta ccagcagccc
      541 cagcagcagc cggtggccca gtcctatggt ggctacaagg agcctgcagc cccagtctcc
      601 atacagcgca gcgccccagg tggtggcggg aagcggtacc gcgtggtgta tgactacagc
      661 gccgccgacg aggacgaggt ctccttccag gacggggaca ccatcgtcaa cgtgcagcag
      721 atcgacgacg gctggatgta cgggacggtg gagcgcaccg gcgacacggg gatgctgccg
      781 gccaactacg tggaggccat cagctccgga ggggatccac cggtcgccac catggtgagc
      841 aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg
      901 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
      961 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg
     1021 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac
     1081 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
     1141 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc
     1201 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag
     1261 aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag
     1321 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag
     1381 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag
     1441 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag
     1501 ggccgccact ccaccggcgg catggacgag ctgtacaagt aaagcggccg cgactctaga
     1561 tcataatcag ccataccaca tt
//
