LOCUS       sequence-85456-e        1336 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Light Chain Myosin, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1336)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1336)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-LC-Myosin-N-7
FEATURES             Location/Qualifiers
     source          1..1336
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      580..589
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             586..1296
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(731..749)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     999..1018
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggactca gatctcgagc tcaagcttat gtcgagcaag
       61 agagccaagg ccaagaccac caagaagagg ccccagaggg ctacgtccaa tgtcttcgcc
      121 atgtttgacc agtcccagat ccaggagttt aaggaggcct tcaacatgat tgatcagaac
      181 cgcgatggct tcattgataa ggaggacctg cacgacatgt tggcctctct ggggaagaac
      241 cccacagacg agtacctgga gggcatgatg aacgaggcgc cagggcccat caacttcacc
      301 atgttcctca caatgtttgg ggagaagctg aacggcacag accccgagga tgtgatccgc
      361 aatgcctttg cctgctttga tgaggaggcc tcaggcttca tccacgagga ccacctgagg
      421 gagctgctca ccaccatggg cgaccgattc acggatgagg aggtggacga gatgtaccgc
      481 gaggcaccca ttgataagaa gggcaacttc aactacgtgg agttcactcg catcctcaaa
      541 cacggcgcca aggacaagga cgacctggat ccaccggtcg ccaccatggt gagcaagggc
      601 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
      661 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
      721 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
      781 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
      841 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
      901 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
      961 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
     1021 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
     1081 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
     1141 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
     1201 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
     1261 cactccaccg gcggcatgga cgagctgtac aagtaaagcg gccgcgactc tagatcataa
     1321 tcagccatac cacatt
//
