LOCUS       sequence-85457-e        2077 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Lysosome Membrane, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2077)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Lysosomes-20
FEATURES             Location/Qualifiers
     source          1..2077
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      40..49
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      44..53
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(46..108,109..1266)
                     /codon_start=1
                     /product="rat lysosome-associated membrane glycoprotein 1"
                     /label=LAMP1
                     /translation="MAAPGARRPLLLLLLAGLAHSAPALFEVKDNNGTACIMASFSASF
                     LTTYEAGHVSKVSNMTLPASAEVLKNSSSCGEKNASEPTLAITFGEGYLLKLTFTKNTT
                     RYSVQHMYFTYNLSDTQFFPNASSKGPDTVDSTTDIKADINKTYRCVSDIRVYMKNVTI
                     VLWDATIQAYLPSSNFSKEETRCPQDQPSPTTGPPSPSPPLVPTNPSVSKYNVTGDNGT
                     CLLASMALQLNITYMKKDNTTVTRAFNINPSDKYSGTCGAQLVTLKVGNKSRVLELQFG
                     MNATSSLFFLQGVQLNMTLPDAIEPTFSTSNYSLKALQASVGNSYKCNSEEHIFVSKAL
                     ALNVFSVQVQAFRVESDRFGSVEECVQDGNNMLIPIAVGGALAGLVLIVLIAYLIGRKR
                     SHAGYQTI"
     regulatory      1321..1330
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1327..2037
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1472..1490)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1740..1759
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cgtcagatcc gctagcgcta ccggactcag atctcgagcg ccaccatggc ggccccgggc
       61 gcccggcggc cgctgctcct gttgctgctg gcaggccttg cacacagcgc cccagcactg
      121 ttcgaggtga aagacaacaa cggcacagcg tgtataatgg ccagcttctc tgcctccttt
      181 ctgaccacct atgaggctgg acatgtttct aaggtctcga atatgaccct gccagcctct
      241 gcagaagtcc tgaagaatag cagctcttgt ggtgaaaaga atgcttctga gcccaccctc
      301 gcaatcacct ttggagaagg atatttactg aaactcacct tcacaaaaaa cacaacacgt
      361 tacagtgtcc agcacatgta tttcacatat aacctgtcag acacacaatt ctttcccaat
      421 gccagctcca aagggcccga cactgtggat tccacaactg acatcaaggc agacatcaac
      481 aaaacatacc gatgtgtcag cgacatcagg gtctacatga agaatgtgac cattgtgctc
      541 tgggacgcta ctatccaggc ctacctgccg agtagcaact tcagcaagga agagacacgc
      601 tgcccacagg atcaaccttc cccaactact gggccaccca gcccctcacc accacttgtg
      661 cccacaaacc ccagtgtgtc caagtacaat gtgactggtg acaatggaac ctgcctgctg
      721 gcctctatgg cactgcaact caacatcacc tacatgaaga aggacaacac gactgtgacc
      781 agagcattca acatcaaccc aagtgacaaa tatagtggga cttgcggtgc ccagttggtg
      841 accctgaagg tggggaacaa gagcagagtc ctggagctgc agtttgggat gaatgccact
      901 tctagcctgt ttttcctgca aggagttcag ttgaacatga ctcttcctga tgccatagag
      961 cccacgttca gcacctccaa ctattccctg aaagctcttc aggccagtgt cggcaactca
     1021 tacaagtgca acagtgagga gcacatcttt gtcagcaagg cgctcgccct caatgtcttc
     1081 agcgtgcaag tccaggcttt cagggtagaa agtgacaggt ttgggtctgt ggaagagtgt
     1141 gtacaggacg gtaacaacat gctgatcccc attgctgtgg gcggggccct ggcagggctg
     1201 gtcctcatcg tcctcatcgc ctacctcatc ggcaggaaga ggagtcacgc gggctatcag
     1261 accatctcgg aattcggctc caccggctcc accggctcca ccggcgcgga tccaccggtc
     1321 gccaccatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc
     1381 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
     1441 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc
     1501 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg
     1561 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
     1621 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
     1681 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
     1741 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag
     1801 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac
     1861 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac
     1921 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag
     1981 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtaaagc
     2041 ggccgcgact ctagatcata atcagccata ccacatt
//
