LOCUS       sequence-85458-e        1136 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Golgi Complex, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1136)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-MannII-N-10
FEATURES             Location/Qualifiers
     source          1..1136
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      386..395
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             392..1102
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(537..555)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     805..824
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tgacgtcaga tccgctagcg ccaccatgaa gttaagtcgc cagttcaccg tgtttggcag
       61 cgcgatcttc tgcgtcgtaa tcttctcact ctacctgatg ctggacaggg gtcacttgga
      121 ctaccctcgg ggcccgcgcc aggagggctc ctttccgcag ggccagcttt caatattgca
      181 agaaaagatt gaccatttgg agcgtttgct cgctgagaac aacgagatca tctcaaatat
      241 cagagactca gtcatcaacc tgagcgagtc tgtggaggac ggcccgcggg ggtcaccagg
      301 caacgccagc caaggctcca tccacctcca ctcgccacag ttggccctgc aggctgaccc
      361 cagctccgga tcggatccac cggtcgccac catggtgagc aagggcgagg aggataacat
      421 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca
      481 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
      541 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt
      601 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct
      661 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
      721 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg
      781 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
      841 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct
      901 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa
      961 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa
     1021 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg
     1081 catggacgag ctgtacaagt aaagcggccg cgactctaga tcataatcag ccatac
//
