LOCUS       sequence-85459-e        2040 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2040)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-MAP4-C-10
FEATURES             Location/Qualifiers
     source          1..2040
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..742
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(180..198)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     448..467
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggaggataa
       61 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
      121 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
      181 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
      241 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
      301 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
      361 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
      421 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
      481 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag
      541 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa
      601 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca
      661 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
      721 cggcatggac gagctgtaca agtccggact cagatctggt ggctccgcag gatcccggca
      781 agaagaagca aaggctgctg taggtgtgac tggaaatgac atcactaccc cgccaaacaa
      841 ggagccacca ccaagcccag aaaagaaagc aaagcctttg gccaccactc aacctgcaaa
      901 gacttcaaca tcgaaagcca aaacacagcc cacttctctc cctaagcaac cagctcccac
      961 cacctctggt gggttgaata aaaaacccat gagcctcgcc tcaggctcag tgccagctgc
     1021 cccacacaaa cgccctgctg ctgccactgc tactgccagg ccttccaccc tacctgccag
     1081 agacgtgaag ccaaagccaa ttacagaagc taaggttgcc gaaaagcgga cctctccatc
     1141 caagccttca tctgccccag ccctcaaacc tggacctaaa accaccccaa ccgtttcaaa
     1201 agccacatct ccctcaactc ttgtttccac tggaccaagt agtagaagtc cagctacaac
     1261 tctgcctaag aggccaacca gcatcaagac tgaggggaaa cctgctgatg tcaaaaggat
     1321 gactgctaag tctgcctcag ctgacttgag tcgctcaaag accacctctg ccagttctgt
     1381 gaagagaaac accactccca ctggggcagc acccccagca gggatgactt ccactcgagt
     1441 caagcccatg tctgcaccta gccgctcttc tggggctctt tctgtggaca agaagcccac
     1501 ttccactaag cctagctcct ctgctcccag ggtgagccgc ctggccacaa ctgtttctgc
     1561 ccctgacctg aagagtgttc gctccaaggt cggctctaca gaaaacatca aacaccagcc
     1621 tggaggaggc cgggccaagg tagagaaaaa aacagaggca gctaccacag ctgggaagcc
     1681 tgaacctaat gcagtcacta aagcagccgg ctccattgcg agtgcacaga aaccgcctgc
     1741 tgggaaagtc cagatagtat ccaaaaaagt gagctacagt catattcaat ccaagtgtgt
     1801 ttccaaggac aatattaagc atgtccctgg atgtggcaat gttcagattc agaacaagaa
     1861 agtggacata tccaaggtct cctccaagtg tgggtccaaa gctaatatca agcacaagcc
     1921 tggtggagga gatgtcaaga ttgaaagtca gaagttgaac ttcaaggaga aggcccaagc
     1981 caaagtggga tagggatcca ccggatctag ataactgatc ataatcagcc ataccacatt
//
