LOCUS       sequence-85460-e        2025 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2025)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-MAP4-N-10
FEATURES             Location/Qualifiers
     source          1..2025
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      15..24
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1269..1278
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1275..1985
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1420..1438)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1688..1707
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tcagatccgc tagcgccacc atggtgtccc ggcaagaaga agcaaaggct gctgtaggtg
       61 tgactggaaa tgacatcact accccgccaa acaaggagcc accaccaagc ccagaaaaga
      121 aagcaaagcc tttggccacc actcaacctg caaagacttc aacatcgaaa gccaaaacac
      181 agcccacttc tctccctaag caaccagctc ccaccacctc tggtgggttg aataaaaaac
      241 ccatgagcct cgcctcaggc tcagtgccag ctgccccaca caaacgccct gctgctgcca
      301 ctgctactgc caggccttcc accctacctg ccagagacgt gaagccaaag ccaattacag
      361 aagctaaggt tgccgaaaag cggacctctc catccaagcc ttcatctgcc ccagccctca
      421 aacctggacc taaaaccacc ccaaccgttt caaaagccac atctccctca actcttgttt
      481 ccactggacc aagtagtaga agtccagcta caactctgcc taagaggcca accagcatca
      541 agactgaggg gaaacctgct gatgtcaaaa ggatgactgc taagtctgcc tcagctgact
      601 tgagtcgctc aaagaccacc tctgccagtt ctgtgaagag aaacaccact cccactgggg
      661 cagcaccccc agcagggatg acttccactc gagtcaagcc catgtctgca cctagccgct
      721 cttctggggc tctttctgtg gacaagaagc ccacttccac taagcctagc tcctctgctc
      781 ccagggtgag ccgcctggcc acaactgttt ctgcccctga cctgaagagt gttcgctcca
      841 aggtcggctc tacagaaaac atcaaacacc agcctggagg aggccgggcc aaggtagaga
      901 aaaaaacaga ggcagctacc acagctggga agcctgaacc taatgcagtc actaaagcag
      961 ccggctccat tgcgagtgca cagaaaccgc ctgctgggaa agtccagata gtatccaaaa
     1021 aagtgagcta cagtcatatt caatccaagt gtgtttccaa ggacaatatt aagcatgtcc
     1081 ctggatgtgg caatgttcag attcagaaca agaaagtgga catatccaag gtctcctcca
     1141 agtgtgggtc caaagctaat atcaagcaca agcctggtgg aggagatgtc aagattgaaa
     1201 gtcagaagtt gaacttcaag gagaaggccc aagccaaagt gggaggcgga ttcgcggatc
     1261 caccggtcgc caccatggtg agcaagggcg aggaggataa catggccatc atcaaggagt
     1321 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg
     1381 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
     1441 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg
     1501 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct
     1561 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact
     1621 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
     1681 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt
     1741 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg
     1801 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg
     1861 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg
     1921 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca
     1981 agtaaagcgg ccgcgactct agatcataat cagccatacc acatt
//
