LOCUS       sequence-85461-e        1869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1869)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-MAPTau-C-10
FEATURES             Location/Qualifiers
     source          1..1869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             29..736
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(174..192)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     442..461
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 agatccgcta gcgctaccgg tcgccaccat ggtgagcaag ggcgaggagg ataacatggc
       61 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga
      121 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
      181 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat
      241 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc
      301 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac
      361 cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg
      421 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
      481 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa
      541 gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc
      601 cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga
      661 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat
      721 ggacgagctg tacaagtccg gaagcggcgg gtccgggagc ggaagcgctg agccccgcca
      781 ggagttcgaa gtgatggaag atcacgctgg gacgtacggg ttgggggaca ggaaagatca
      841 ggggggctac accatgcacc aagaccaaga gggtgacacg gacgctggcc tgaaagctga
      901 agaagcaggc attggagaca cccccagcct ggaagacgaa gctgctggtc acgtgaccca
      961 agctcgcatg gtcagtaaaa gcaaagacgg gactggaagc gatgacaaaa aagccaaggg
     1021 ggctgatggt aaaacgaaga tcgccacacc gcggggagca gcccctccag gccagaaggg
     1081 ccaggccaac gccaccagga ttccagcaaa aaccccgccc gctccaaaga caccacccag
     1141 ctctggtgaa cctccaaaat caggggatcg cagcggctac agcagccccg gctccccagg
     1201 cactcccggc agccgctccc gcaccccgtc ccttccaacc ccacccaccc gggagcccaa
     1261 gaaggtggca gtggtccgta ctccacccaa gtcgccgtct tccgccaaga gccgcctgca
     1321 gacagccccc gtgcccatgc cagacctgaa gaatgtcaag tccaagatcg gctccactga
     1381 gaacctgaag caccagccgg gaggcgggaa ggtgcaaata gtctacaaac cagttgacct
     1441 gagcaaggtg acctccaagt gtggctcatt aggcaacatc catcataaac caggaggtgg
     1501 ccaggtggaa gtaaaatctg agaagcttga cttcaaggac agagtccagt cgaagattgg
     1561 gtccctggac aatatcaccc acgtccctgg cggaggaaat aaaaagattg aaacccacaa
     1621 gctgaccttc cgcgagaacg ccaaagccaa gacagaccac ggggcggaga tcgtgtacaa
     1681 gtcgccagtg gtgtctgggg acacgtctcc acggcatctc agcaatgtct cctccaccgg
     1741 cagcatcgac atggtagact cgccccagct cgccacgcta gctgacgagg tgtctgcctc
     1801 cctggccaag cagggtttgt gaggatccac cggatctaga taactgatca taatcagcca
     1861 taccacatt
//
