LOCUS       sequence-85463-e        1711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin Capping Protein , Excitation: 587, Emission: 
            610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1711)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-MCPA2-C-18
FEATURES             Location/Qualifiers
     source          1..1711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             29..736
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(174..192)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     442..461
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 agatccgcta gcgctaccgg tcgccaccat ggtgagcaag ggcgaggagg ataacatggc
       61 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga
      121 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
      181 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat
      241 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc
      301 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac
      361 cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg
      421 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
      481 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa
      541 gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc
      601 cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga
      661 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat
      721 ggacgagctg tacaagtccg gactcagatc tagtagcgga ggtagcgcat ccggcggaag
      781 cggaggaagc gcggatctgg aggagcagtt gtccgatgag gagaaggttc gtatagcagc
      841 aaaattcatc attcacgccc ctcctggaga atttaatgag gtttttaatg atgttcgact
      901 attgcttaat aatgataatc ttctcaggga aggagccgca catgcatttg cacagtataa
      961 cctggaccag tttacaccag taaaaattga aggctatgaa gatcaggttt tgataacaga
     1021 acatggagac ttgggaaatg gaaaattttt ggatccaaaa aacagaatct gtttcaaatt
     1081 tgatcattta agaaaggaag caactgatcc aaggccctac gaagcagaaa atgccattga
     1141 atcctggaga acttcagtag aaactgcact gcgagcttat gtaaaagagc attatccgaa
     1201 tggggtctgc actgtgtatg gcaaaaaagt agatgggcag caaaccatta ttgcatgcat
     1261 agaaagccat cagtttcaag caaaaaactt ttggaatggt cgttggaggt cagagtggaa
     1321 gtttacagtc actccttcca ccacacaggt ggttggcatt ttgaaaattc aggttcatta
     1381 ttatgaagat ggtaatgttc agctagtgag tcataaagat atacaagatt ccctcacagt
     1441 atctaatgag gtgcaaacag caaaagaatt tataaagatt gtagaagctg cagaaaatga
     1501 ataccagact gccatcagtg agaattatca gacgatgtcg gacactacct tcaaagcctt
     1561 acgtcgacag ttgccagtga cacgtactaa gatcgactgg aacaagatcc ttagctacaa
     1621 gattggcaaa gagatgcaga atgcataaga attctgcagt cgacggtacc gcgggcccgg
     1681 gatccaccgg atctagataa ctgatcataa t
//
