LOCUS       sequence-85465-e        1689 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin Capping Protein , Excitation: 587, Emission: 
            610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1689)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-MCPB1-C-18
FEATURES             Location/Qualifiers
     source          1..1689
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             33..740
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(178..196)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     446..465
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cgtcagatcc gctagcgcta ccggtcgcca ccatggtgag caagggcgag gaggataaca
       61 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc
      121 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
      181 agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt
      241 tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc
      301 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg
      361 tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc
      421 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg
      481 cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc
      541 tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga
      601 agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca
      661 acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg
      721 gcatggacga gctgtacaag tccggactca gatctcgaga gtccagtgga tattcgtctc
      781 ggtcgggtgg aagcagcgat cagcagctgg actgcgcctt ggacctgatg aggcgcctgc
      841 ctccacagca gattgagaag aacctcagcg atctgatcga cctggtcccc agtctgtgtg
      901 aagatctcct gtcatctgtt gaccagcccc tgaaaattgc cagagacaag gtggtgggca
      961 aggattacct tttgtgtgac tacaacagag acggggactc ctataggtca ccgtggagta
     1021 acaagtatga ccctcctttg gaagatgggg ccatgccatc tgctcggctc agaaagctgg
     1081 aggtagaggc caacaatgcc ttcgaccaat accgagacct gtattttgaa ggtggggtct
     1141 catcagtcta cctctgggat cttgatcatg gctttgctgg agtgatcctc ataaagaaag
     1201 ctggagatgg atccaagaag atcaaaggct gctgggattc catccacgtg gtggaagtgc
     1261 aggagaagtc cagcggccgt actgcccatt acaagttgac ctccacggtg atgctatggc
     1321 tgcaaaccaa caaatccggc tcgggcacca tgaacctggg aggcagccta accagacaga
     1381 tggagaaaga cgaaactgtg agtgactgtt ccccacacat agccaacatc gggcgcctgg
     1441 tggaggacat ggaaaacaaa atccgaagca cgctgaatga gatctacttt ggaaaaacaa
     1501 aggacatcgt caacgggctg agatctcttg atgctatccc cgacaaccac aagtttaagc
     1561 agttgcagag ggaactttct caagtgctga cccagcgcca ggtctacatc cagcctgata
     1621 attgagaatt ctgcagtcga cggtaccgcg ggcccgggat ccaccggatc tagataactg
     1681 atcataatc
//
