LOCUS       sequence-85466-e        1653 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin Capping Protein , Excitation: 587, Emission: 
            610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1653)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-MCPB1-N-18
FEATURES             Location/Qualifiers
     source          1..1653
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      903..912
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             909..1619
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1054..1072)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1322..1341
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgcc accatgagcg atcagcagct ggactgcgcc ttggacctga
       61 tgaggcgcct gcctccacag cagattgaga agaacctcag cgatctgatc gacctggtcc
      121 ccagtctgtg tgaagatctc ctgtcatctg ttgaccagcc cctgaaaatt gccagagaca
      181 aggtggtggg caaggattac cttttgtgtg actacaacag agacggggac tcctataggt
      241 caccgtggag taacaagtat gaccctcctt tggaagatgg ggccatgcca tctgctcggc
      301 tcagaaagct ggaggtagag gccaacaatg ccttcgacca ataccgagac ctgtattttg
      361 aaggtggggt ctcatcagtc tacctctggg atcttgatca tggctttgct ggagtgatcc
      421 tcataaagaa agctggagat ggatccaaga agatcaaagg ctgctgggat tccatccacg
      481 tggtggaagt gcaggagaag tccagcggcc gtactgccca ttacaagttg acctccacgg
      541 tgatgctatg gctgcaaacc aacaaatccg gctcgggcac catgaacctg ggaggcagcc
      601 taaccagaca gatggagaaa gacgaaactg tgagtgactg ttccccacac atagccaaca
      661 tcgggcgcct ggtggaggac atggaaaaca aaatccgaag cacgctgaat gagatctact
      721 ttggaaaaac aaaggacatc gtcaacgggc tgagatctct tgatgctatc cccgacaacc
      781 acaagtttaa gcagttgcag agggaacttt ctcaagtgct gacccagcgc caggtctaca
      841 tccagcctga taatagtcgc ggaggcagtg gtggggtacc gcgggcccgg gatccaccgg
      901 tcgccaccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc
      961 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg
     1021 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc
     1081 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg
     1141 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt
     1201 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc
     1261 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg
     1321 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg
     1381 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact
     1441 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct
     1501 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac
     1561 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtaaa
     1621 gcggccgcga ctctagatca taatcagcca tac
//
