LOCUS       sequence-85467-e        1679 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin Capping Protein , Excitation: 587, Emission: 
            610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1679)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-MCPB2-C-18
FEATURES             Location/Qualifiers
     source          1..1679
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..742
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(180..198)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     448..467
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggaggataa
       61 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
      121 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
      181 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
      241 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
      301 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
      361 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
      421 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
      481 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag
      541 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa
      601 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca
      661 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
      721 cggcatggac gagctgtaca agtccggact cagatctcga gagtccagtg gatattcgtc
      781 tcggtcgggt ggaagcagcg atcagcagct ggactgcgcc ttggacctga tgaggcgcct
      841 gcctccacag cagattgaga agaacctcag cgatctgatc gacctggtcc ccagtctgtg
      901 tgaagatctc ctgtcatctg ttgaccagcc cctgaaaatt gccagagaca aggtggtggg
      961 caaggattac cttttgtgtg actacaacag agacggggac tcctataggt caccgtggag
     1021 taacaagtat gaccctcctt tggaagatgg ggccatgcca tctgctcggc tcagaaagct
     1081 ggaggtagag gccaacaatg ccttcgacca ataccgagac ctgtattttg aaggtggggt
     1141 ctcatcagtc tacctctggg atcttgatca tggctttgct ggagtgatcc tcataaagaa
     1201 agctggagat ggatccaaga agatcaaagg ctgctgggat tccatccacg tggtggaagt
     1261 gcaggagaag tccagcggcc gtactgccca ttacaagttg acctccacgg tgatgctatg
     1321 gctgcaaacc aacaaatccg gctcgggcac catgaacctg ggaggcagcc taaccagaca
     1381 gatggagaaa gacgaaactg tgagtgactg ttccccacac atagccaaca tcgggcgcct
     1441 ggtggaggac atggaaaaca aaatccgaag cacgctgaat gagatctact ttggaaaaac
     1501 aaaggacatc gtcaacgggc tgaggtctgt gcagacgttt gcagacaaat caaagcaaga
     1561 agcgcttaag aacgacctgg tggaggcctt gaagagaaag cagcagtgtt gagaattctg
     1621 cagtcgacgg taccgcgggc ccgggatcca ccggatctag ataactgatc ataatcagc
//
