LOCUS       sequence-85468-e        1643 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin Capping Protein , Excitation: 587, Emission: 
            610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1643)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-MCPB2-N-18
FEATURES             Location/Qualifiers
     source          1..1643
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      890..899
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             896..1606
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1041..1059)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1309..1328
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tgacgtcaga tccgctagcg ccaccatgag cgatcagcag ctggactgcg ccttggacct
       61 gatgaggcgc ctgcctccac agcagattga gaagaacctc agcgatctga tcgacctggt
      121 ccccagtctg tgtgaagatc tcctgtcatc tgttgaccag cccctgaaaa ttgccagaga
      181 caaggtggtg ggcaaggatt accttttgtg tgactacaac agagacgggg actcctatag
      241 gtcaccgtgg agtaacaagt atgaccctcc tttggaagat ggggccatgc catctgctcg
      301 gctcagaaag ctggaggtag aggccaacaa tgccttcgac caataccgag acctgtattt
      361 tgaaggtggg gtctcatcag tctacctctg ggatcttgat catggctttg ctggagtgat
      421 cctcataaag aaagctggag atggatccaa gaagatcaaa ggctgctggg attccatcca
      481 cgtggtggaa gtgcaggaga agtccagcgg ccgtactgcc cattacaagt tgacctccac
      541 ggtgatgcta tggctgcaaa ccaacaaatc cggctcgggc accatgaacc tgggaggcag
      601 cctaaccaga cagatggaga aagacgaaac tgtgagtgac tgttccccac acatagccaa
      661 catcgggcgc ctggtggagg acatggaaaa caaaatccga agcacgctga atgagatcta
      721 ctttggaaaa acaaaggaca tcgtcaacgg gctgaggtct gtgcagacgt ttgcagacaa
      781 atcaaagcaa gaagcgctta agaacgacct ggtggaggcc ttgaagagaa agcagcagtg
      841 tagtcgcgga ggcagtggtg gggtaccgcg ggcccgggat ccaccggtcg ccaccatggt
      901 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca
      961 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     1021 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
     1081 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc
     1141 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     1201 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga
     1261 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
     1321 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
     1381 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
     1441 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
     1501 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
     1561 cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtaaagcg gccgcgactc
     1621 tagatcataa tcagccatac caa
//
