LOCUS       sequence-85469-e         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Olenych SG, Claxton NS, Ottenberg GK, Davidson MW
  TITLE     The fluorescent protein color palette.
  JOURNAL   Curr Protoc Cell Biol. 2007 Sep;Chapter 21:Unit 21.5. doi: 
            10.1002/0471143030.cb2105s36.
  PUBMED    18228502
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Protoc
            Cell Biol."; date: "2007-09"; volume: "Chapter 21:Unit 21.5. doi"; 
            pages: " 10.1002/0471143030.cb2105s36"
COMMENT     Sequence Label: mCherry-Mito-7
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             19..93
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      121..130
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             127..837
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(272..290)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     540..559
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcat gtccgtcctg acgccgctgc tgctgcgggg cttgacaggc
       61 tcggcccggc ggctcccagt gccgcgcgcc aagatccatt cgttggggga tccaccggtc
      121 gccaccatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc
      181 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
      241 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc
      301 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg
      361 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
      421 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
      481 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
      541 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag
      601 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac
      661 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac
      721 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag
      781 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtaaagc
      841 ggccgcgact ctagatcata atcagcatac cacatttgta gaggttttac ttgctttaaa
      901 aacctcccac acctcccctg aacctgaaca taaatgatgc aattgttgtg tacttgttat
      961 ncagctta
//
