LOCUS       sequence-85471-e        2532 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton/Membrane, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2532)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Moesin-N-14
FEATURES             Location/Qualifiers
     source          1..2532
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1791..1800
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1797..2507
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1942..1960)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2210..2229
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgcc accatgccca aaacgatcag tgtgcgtgtg accaccatgg
       61 atgcagagct ggagtttgcc atccagccca acaccaccgg gaagcagcta tttgaccagg
      121 tggtgaaaac tattggcttg agggaagttt ggttctttgg tctgcagtac caggacacta
      181 aaggtttctc cacctggctg aaactcaata agaaggtgac tgcccaggat gtgcggaagg
      241 aaagccccct gctctttaag ttccgtgcca agttctaccc tgaggatgtg tccgaggaat
      301 tgattcagga catcactcag cgcctgttct ttctgcaagt gaaagagggc attctcaatg
      361 atgatattta ctgcccgcct gagaccgctg tgctgctggc ctcgtatgct gtccagtcta
      421 agtatggcga cttcaataag gaagtgcata agtctggcta cctggccgga gacaagttgc
      481 tcccgcagag agtcctggaa cagcacaaac tcaacaagga ccagtgggag gagcggatcc
      541 aggtgtggca tgaggaacac cgtggcatgc tcagggagga tgctgtcctg gaatatctga
      601 agattgctca agatctggag atgtatggtg tgaactactt cagcatcaag aacaagaaag
      661 gctcagagct gtggctgggg gtggatgccc tgggtctcaa catctatgag cagaatgaca
      721 gactaactcc caagataggc ttcccctgga gtgaaatcag gaacatctct ttcaatgata
      781 agaaatttgt catcaagccc attgacaaaa aagccccgga cttcgtcttc tatgctcccc
      841 ggctgcggat taacaagcgg atcttggcct tgtgcatggg gaaccatgaa ctatacatgc
      901 gccgtcgcaa gcctgatacc attgaggtgc agcagatgaa ggcacaggcc cgggaggaga
      961 agcaccagaa gcagatggag cgtgctatgc tggaaaatga gaagaagaag cgtgaaatgg
     1021 cagagaagga gaaagagaag attgaacggg agaaggagga gctgatggag aggctgaagc
     1081 agatcgagga acagactaag aaggctcagc aagaactgga agaacagacc cgtagggctc
     1141 tggaacttga gcaggaacgg aagcgtgccc agagcgaggc tgaaaagctg gccaaggagc
     1201 gtcaagaagc tgaagaggcc aaggaggcct tgctgcaggc ctcccgggac cagaaaaaga
     1261 ctcaggaaca gctggccttg gaaatggcag agctgacagc tcgaatctcc cagctggaga
     1321 tggcccgaca gaagaaggag agtgaggctg tggagtggca gcagaaggcc cagatggtac
     1381 aggaagactt ggagaagacc cgtgctgagc tgaagactgc catgagtaca cctcatgtgg
     1441 cagagcctgc tgagaatgag caggatgagc aggatgagaa tggggcagag gctagtgctg
     1501 acctacgggc tgatgctatg gccaaggacc gcagtgagga ggaacgtacc actgaggcag
     1561 agaagaatga gcgtgtgcag aagcacctga aggccctcac ttcggagctg gccaatgcca
     1621 gagatgagtc caagaagact gccaatgaca tgatccatgc tgagaacatg cgactgggcc
     1681 gagacaaata caagaccctg cgccagatcc ggcagggcaa caccaagcag cgcattgacg
     1741 aatttgagtc tatggcatcc ggcggtagcg gcggtggcag cggaccggtc gccaccatgg
     1801 tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc
     1861 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     1921 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg
     1981 cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg
     2041 ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     2101 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg
     2161 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc
     2221 agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc
     2281 tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg
     2341 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca
     2401 tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg
     2461 ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtaaagc ggccgcgact
     2521 ctagatcata at
//
