LOCUS       sequence-85474-e        6787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6787)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 6787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-MyosinIIB-N-18
FEATURES             Location/Qualifiers
     source          1..6787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      61..70
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      6043..6052
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6049..6759
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6194..6212)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6462..6481
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gctagcgcta ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc
       61 gccaccatgg cgcagagaac tggactcgag gatccagaga ggtatctctt tgtggacagg
      121 gctgtcatct acaaccctgc cactcaagct gattggacag ctaaaaagct agtgtggatt
      181 ccatcagaac gccatggttt tgaggcagct agtatcaaag aagaacgggg agatgaagtt
      241 atggtggagt tggcagagaa tggaaagaaa gcaatggtca acaaagatga tattcagaag
      301 atgaacccac ctaagttttc caaggtggag gatatggcag aattgacatg cttgaatgaa
      361 gcttccgttt tacataatct gaaggatcgc tactattcag gactaatcta tacttattct
      421 ggactcttct gtgtagttat aaacccttac aagaatcttc caatttactc tgagaatatt
      481 attgaaatgt acagagggaa gaagcgtcat gagatgcctc cacacatcta tgctatatct
      541 gaatctgctt acagatgcat gcttcaagat cgtgaggacc agtcaattct ttgcacgggt
      601 gagtcaggtg ctgggaagac agaaaataca aagaaagtta ttcagtacct tgcccatgtt
      661 gcttcttcac ataaaggaag aaaggaccat aatattcctg gggaacttga acggcagctt
      721 ttgcaagcaa atccaattct ggaatcattt ggaaatgcga agactgtgaa aaatgataac
      781 tcatctcgtt ttggcaaatt tattcggatc aactttgatg taactggcta tatcgttggg
      841 gccaacattg aaacatacct tctggaaaag tctcgtgctg ttcgtcaagc aaaagatgaa
      901 cgtacttttc atatctttta ccagttgtta tctggagcag gagaacacct aaagtctgat
      961 ttgcttcttg aaggatttaa taactacagg tttctctcca atggctatat tcctattccg
     1021 ggacagcaag acaaagataa tttccaggag accatggaag caatgcacat aatgggcttc
     1081 tcccatgaag agattctgtc aatgcttaaa gtagtatctt cagtgctaca gtttggaaat
     1141 atttctttca aaaaggagag aaatactgat caagcttcca tgccagaaaa tacagttgcg
     1201 cagaagctct gccatcttct tgggatgaat gtgatggagt ttactcgggc catcctgact
     1261 ccccggatca aggtcggccg agactatgtg caaaaagccc agaccaaaga acaggcagat
     1321 tttgcagtag aagcattggc aaaagctacc tatgagcggc tctttcgctg gctcgttcat
     1381 cgcatcaata aagctctgga taggaccaaa cgtcagggag catctttcat tggaatcctg
     1441 gatattgctg gatttgaaat ttttgagctg aactcctttg aacaactttg catcaactac
     1501 accaatgaga agctgcagca gctgttcaac cacaccatgt ttatcctaga acaagaggaa
     1561 taccagcgcg aaggcatcga gtggaacttc atcgatttcg ggctggatct gcagccatgc
     1621 atcgacctaa tagagagacc tgcgaaccct cctggtgtac tggccctttt ggatgaagaa
     1681 tgctggttcc ctaaagccac agataaaacc tttgttgaaa aactggttca agagcaaggt
     1741 tcccactcca agtttcagaa acctcgacaa ttaaaagaca aagctgattt ttgcattata
     1801 cattatgcag ggaaggtgga ctataaggca gatgagtggc tgatgaagaa tatggacccc
     1861 ctgaatgaca acgtggccac ccttttgcac cagtcatcag acagatttgt ggcagagctt
     1921 tggaaagatg tggaccgtat cgtgggtctg gatcaagtca ctggtatgac tgagacagct
     1981 tttggctccg catataaaac caagaagggc atgtttcgta ccgttgggca actctacaaa
     2041 gaatctctca ccaagctgat ggcaactctc cgaaacacca accctaactt tgttcgttgt
     2101 atcattccaa atcacgagaa gagggctgga aaattggatc cacacctagt cctagatcag
     2161 cttcgctgta atggtgtcct ggaagggatc cgaatctgtc gccagggctt ccctaaccga
     2221 atagttttcc aggaattcag acagagatat gagatcctaa ctccaaatgc tattcctaaa
     2281 ggttttatgg atggtaaaca ggcctgtgaa cgaatgatcc gggctttaga attggaccca
     2341 aacttgtaca gaattggaca gagcaagata tttttcagag ctggagttct ggcacactta
     2401 gaggaagaaa gagatttaaa aatcaccgat atcattatct tcttccaggc cgtttgcaga
     2461 ggttacctgg ccagaaaggc ctttgccaag aagcagcagc aactaagtgc cttaaaggtc
     2521 ttgcagcgga actgtgccgc gtacctgaaa ttacggcact ggcagtggtg gcgagtcttc
     2581 acaaaggtga agccgcttct acaagtgact cgccaggagg aagaacttca ggccaaagat
     2641 gaagagctgt tgaaggtgaa ggagaagcag acaaaggtgg aaggagagct ggaggagatg
     2701 gagcggaagc accagcagct tttagaagag aagaatatcc ttgcagaaca actacaagca
     2761 gagactgagc tctttgctga agcagaagag atgagggcaa gacttgctgc taaaaagcag
     2821 gaattagaag agattctaca tgacttggag tctagggttg aagaagaaga agaaagaaac
     2881 caaatcctcc aaaatgaaaa gaaaaaaatg caagcacata ttcaggacct ggaagaacag
     2941 ctagacgagg aggaaggggc tcggcaaaag ctgcagctgg aaaaggtgac agcagaggcc
     3001 aagatcaaga agatggaaga ggagattctg cttctcgagg accaaaattc caagttcatc
     3061 aaagaaaaga aactcatgga agatcgcatt gctgagtgtt cctctcagct ggctgaagag
     3121 gaagaaaagg cgaaaaactt ggccaaaatc aggaataagc aagaagtgat gatctcagat
     3181 ctagaagaac gcttaaagaa ggaagaaaag actcgtcagg aactggaaaa ggccaaaaga
     3241 aaactcgacg gggagacgac cgacctgcag gaccagatcg cagagctgca ggcgcagatt
     3301 gatgagctca agctgcagct ggccaagaag gaggaggagc tgcagggcgc actggccaga
     3361 ggtgatgatg aaacactcca taagaacaat gcccttaaag ttgtgcgaga gctacaagcc
     3421 caaattgctg aacttcagga agactttgaa tccgagaagg cttcacggaa caaggccgaa
     3481 aagcagaaaa gggacttgag tgaggaactg gaagctctga aaacagagct ggaggacacg
     3541 ctggacacca cggcagccca gcaggaacta cgtacaaaac gtgaacaaga agtggcagag
     3601 ctgaagaaag ctcttgagga ggaaactaag aaccatgaag ctcaaatcca ggacatgaga
     3661 caaagacacg caacagccct ggaggagctc tcagagcagc tggaacaggc caagcggttc
     3721 aaagcaaatc tagagaagaa caagcagggc ctggagacag ataacaagga gctggcgtgt
     3781 gaggtgaagg tcctgcagca ggtcaaggct gagtctgagc acaagaggaa gaagctcgac
     3841 gcgcaggtcc aggagctcca tgccaaggtc tctgaaggcg acaggctcag ggtggagctg
     3901 gcggagaaag caagtaagct gcagaatgag ctagataatg tctccaccct tctggaagaa
     3961 gcagagaaga agggtattaa atttgctaag gatgcagcta gtcttgagtc tcaactacag
     4021 gatacacagg agcttcttca ggaggagaca cgccagaaac taaacctgag cagtcggatc
     4081 cggcagctgg aagaggagaa gaacagtctt caggagcagc aggaggagga ggaggaggcc
     4141 aggaagaacc tggagaagca agtgctggcc ctgcagtccc agttggctga taccaagaag
     4201 aaagtagatg acgacctggg aacaattgaa agtctggaag aagccaagaa gaagcttctg
     4261 aaggacgcgg aggccctgag ccagcgcctg gaggagaagg cactggcgta tgacaaactg
     4321 gagaagacca agaaccgcct gcagcaggag ctggacgacc tcacggtgga cctggaccac
     4381 cagcgccagg tcgcctccaa cttggagaag aagcagaaga agtttgacca gctgttagca
     4441 gaagagaaga gcatctctgc tcgctatgcc gaagagcggg accgggccga agccgaggcc
     4501 agagagaaag aaaccaaagc cctgtcactg gcccgggccc tcgaggaagc cctggaggcc
     4561 aaggaggagt ttgagaggca gaacaagcag ctccgagcag acatggaaga cctcatgagc
     4621 tccaaagatg atgtgggaaa aaacgttcac gaacttgaaa aatccaaacg ggccctagag
     4681 cagcaggtgg aggaaatgag gacccagctg gaggagctgg aagacgaact ccaggccacg
     4741 gaagatgcca agcttcgtct ggaggtcaac atgcaggcca tgaaggcgca gttcgagaga
     4801 gacctgcaaa ccagggatga gcagaatgaa gagaagaagc ggctgctgat caaacaggtg
     4861 cgggagctcg aggcggagct ggaggatgag aggaaacagc gggcgcttgc tgtagcttca
     4921 aagaaaaaga tggagataga cctgaaggac ctcgaagccc aaatcgaggc tgcgaacaaa
     4981 gctcgggatg aggtgattaa gcagctccgc aagctccagg ctcagatgaa ggattaccaa
     5041 cgtgaattag aagaagctcg tgcatccaga gatgagattt ttgctcaatc caaagagagt
     5101 gaaaagaaat tgaagagtct ggaagcagaa atccttcaat tgcaggagga acttgcctca
     5161 tctgagcgag cccgccgaca cgccgagcag gagagagatg agctggcgga cgagatcacc
     5221 aacagcgcct ctggcaagtc cgcgctgctg gatgagaagc ggcgtctgga agctcggatc
     5281 gcacagctgg aggaggagct ggaagaggag cagagcaaca tggagctgct caacgaccgc
     5341 ttccgcaaga ccactctaca ggtggacaca ctgaacgccg agctagcagc cgagcgcagc
     5401 gccgcccaga agagtgacaa tgcacgccag caactggagc ggcagaacaa ggagctgaag
     5461 gccaagctgc aggaactcga gggtgctgtc aagtctaagt tcaaggccac catctcagcc
     5521 ctggaggcca agattgggca gctggaggag cagcttgagc aggaagccaa ggaacgagca
     5581 gccgccaaca aattagtccg tcgcactgag aagaagctga aagaaatctt catgcaggtt
     5641 gaggatgagc gtcgacacgc ggaccagtat aaagagcaga tggagaaggc caacgctcgg
     5701 atgaagcagc ttaaacgcca gctggaggaa gcagaagaag aagcgacgcg tgccaacgca
     5761 tctcggcgta aactccagcg ggaactggat gatgccaccg aggccaacga gggcctgagc
     5821 cgcgaggtca gcaccctgaa gaaccggctg aggcggggtg gccccatcag cttctcttcc
     5881 agccgatctg gccggcgcca gctgcacctt gaaggagctt ccctggagct ctccgacgat
     5941 gacacagaaa gtaagaccag tgatgtcaac gagacgcagc caccccagtc agagggcagc
     6001 ggtggaggca gcgcatccgg cggaagcgga agcggaccgg tcgccaccat ggtgagcaag
     6061 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
     6121 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     6181 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
     6241 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
     6301 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     6361 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
     6421 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
     6481 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
     6541 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
     6601 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg
     6661 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
     6721 cgccactcca ccggcggcat ggacgagctg tacaagtaaa gcggccgcga ctctagatca
     6781 taatcag
//
