LOCUS       sequence-85479-e        2372 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tight Junctions, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2372)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Occludin-N-10
FEATURES             Location/Qualifiers
     source          1..2372
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             52..60
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     regulatory      1616..1625
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1622..2332
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1767..1785)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2035..2054
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tgacgtcaga tccgctagcg ccaccatgtc atccaggcct cttgaaagtc cacctcctta
       61 caggcctgat gaattcaaac cgaatcatta tgcaccaagc aatgacatat atggtggaga
      121 gatgcatgtt cgaccaatgc tctctcagcc agcctactct ttttacccag aagatgaaat
      181 tcttcacttc tacaaatgga cctctcctcc aggagtgatt cggatcctgt ctatgctcat
      241 tattgtgatg tgcattgcca tctttgcctg tgtggcctcc acgcttgcct gggacagagg
      301 ctatggaact tcccttttag gaggtagtgt aggctaccct tatggaggaa gtggctttgg
      361 tagctacgga agtggctatg gctatggcta tggttatggc tatggctacg gaggctatac
      421 agacccaaga gcagcaaagg gcttcatgtt ggccatggct gccttttgtt tcattgccgc
      481 gttggtgatc tttgttacca gtgttataag atctgaaatg tccagaacaa gaagatacta
      541 cttaagtgtg ataatagtga gtgctatcct gggcatcatg gtgtttattg ccacaattgt
      601 ctatataatg ggagtgaacc caactgctca gtcttctgga tctctatatg gttcacaaat
      661 atatgccctc tgcaaccaat tttatacacc tgcagctact ggactctacg tggatcagta
      721 tttgtatcac tactgtgttg tggatcccca ggaggccatt gccattgtac tggggttcat
      781 gattattgtg gcttttgctt taataatttt ctttgctgtg aaaactcgaa gaaagatgga
      841 caggtatgac aagtccaata ttttgtggga caaggaacac atttatgatg agcagccccc
      901 caatgtcgag gagtgggtta aaaatgtgtc tgcaggcaca caggacgtgc cttcaccccc
      961 atctgactat gtggaaagag ttgacagtcc catggcatac tcttccaatg gcaaagtgaa
     1021 tgacaagcgg ttttatccag agtcttccta taaatccacg ccggttcctg aagtggttca
     1081 ggagcttcca ttaacttcgc ctgtggatga cttcaggcag cctcgttaca gcagcggtgg
     1141 taactttgag acaccttcaa aaagagcacc tgcaaaggga agagcaggaa ggtcaaagag
     1201 aacagagcaa gatcactatg agacagacta cacaactggc ggcgagtcct gtgatgagct
     1261 ggaggaggac tggatcaggg aatatccacc tatcacttca gatcaacaaa gacaactgta
     1321 caagaggaat tttgacactg gcctacagga atacaagagc ttacaatcag aacttgatga
     1381 gatcaataaa gaactctccc gtttggataa agaattggat gactatagag aagaaagtga
     1441 agagtacatg gctgctgctg atgaatacaa tagactgaag caagtgaagg gatctgcaga
     1501 ttacaaaagt aagaagaatc attgcaagca gttaaagagc aaattgtcac acatcaagaa
     1561 gatggttgga gactatgata gacagaaaac aagcggctcc ggtagcggac cggtcgccac
     1621 catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa
     1681 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     1741 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc
     1801 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca
     1861 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg
     1921 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
     1981 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
     2041 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg
     2101 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc
     2161 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt
     2221 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
     2281 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt aaagcggccg
     2341 cgactctaga tcataatcag ccataccaca tt
//
