LOCUS       sequence-85480-e        2840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2840)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Palladin-C-7
FEATURES             Location/Qualifiers
     source          1..2840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             39..746
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(184..202)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     452..471
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tagtgacgtc agatccgcta gcgctaccgg tcgccaccat ggtgagcaag ggcgaggagg
       61 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga
      121 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
      181 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
      241 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
      301 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
      361 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga
      421 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct
      481 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc
      541 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg
      601 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct
      661 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca
      721 ccggcggcat ggacgagctg tacaagtccg gactcagatc tcgagtgagc gctctggcct
      781 cccgcgctac cgccatgcag tcctccggct ccttcaacta cgcgcgccct aagcagttca
      841 tcgcggcgca gaacctgggt cccgcatcgg ggctgcccac gccaacctcc agccccagct
      901 cctccagctt gccgtcacca ttgtccccca cgcccaggcc cttcggccgc gcgcccgggc
      961 cgcccttcgt ggaacccgag gccatgtggg gcccgtcctc gccctcgccg ccaccgccgc
     1021 cacctccggt cttcagcccc tctgcagcct acccggtgcc cgacgtgttc ccactgccac
     1081 cgccgccgcc accgctacct agctccacct cacactgcgc ctcgcccgcc cgcttcggcc
     1141 ccagccagac gcccgcagcc ttcctcagcg ccctgctgcc ctctcagcca ccgcctgtcg
     1201 ctgtcaacgc cctggggctg cccaagggag tcacccccgc ggggttccca aagaagtcca
     1261 gtagaactgc tagaattgcc tctgatgagg agattcaagg cacaaaggat gctgtcatcc
     1321 aagacctgga acggaagctt cgcttcaagg aggaccttct gaacaatggc caaccgaggc
     1381 taacctatga ggaaagaatg gctcgccgcc tgcttggagc cgacagcgca aacgtcttca
     1441 acatccagga gccagaggaa acggcagcca atcaggagta caaagtctct agctgcgagc
     1501 agaggctgat tagcgagatt gaatacaggc tggagcgctc ccctgtggat gagtcgggag
     1561 acgaggtgca ggatccagat gtgcctgtgg agaacgcaac agctcccttc tttgagatga
     1621 agctgaaaca ctacaagatc tttgagggga tgccggtgac tttcacgtgt cgagtggctg
     1681 ggaatccaaa gccaaagatc tattggttta aagatgggaa gcagatttct ccgaagagcg
     1741 atcactacac cattcagaga gaccttgatg ggacctgctc tctccacacc acggcctcta
     1801 ccctagacga cgatgggaac tacaccatca tggctgccaa ccctcagggt cgcgtcagtt
     1861 gtacaggaag gctaatggta caggctgtca accaaagagg ccgcagtccc cgctctccct
     1921 caggccatcc tcatgccaga aggcctcgct ctcgatcacg ggacagtgga gatgaaaacg
     1981 agcccattca ggagcgattc ttcagacctc acttcctgca ggctcctgga gacctgaccg
     2041 ttcaggaagg caagctctgc aggatggact gcaaggtcag tgggttacca accccagatc
     2101 tcagctggca actagacgga aagcccatac gccccgacag tgctcacaag atgctggtcc
     2161 gtgagaatgg ggtccactcc ctcattatag agccagtcac gtcccgggac gccggcatct
     2221 acacatgtat tgccaccaac agagcaggac agaactcgtt taacctggag cttgtggttg
     2281 ctgctaagga agcacacaag gcccctgtgt ttatggagaa gctacagaac acgggggttg
     2341 ctgatggata cccagtgcgg ctagaatgcc gtgtctccgg agtgccgcca cctcagatat
     2401 tttggaagaa agaaaatgaa tcgctcactc atagcactga gcgagtaagc atgcaccagg
     2461 ataatcatgg ctacatctgc ctgctcatcc agggagccac aaaggaagac gctgggtggt
     2521 acactgtgtc tgccaagaac gaagcaggca ttgtgtcctg cactgcccgg ctggatgtct
     2581 acacccagtg gcaccagcag ccacagacca ccaagccaaa aaaagtacgg ccctcggcca
     2641 gtcgctacgc agcactttcg gaccagggac tagacatcaa agccgctttc caacctgaag
     2701 ccagcccatc tcacctgact ctgaacagcg gcttggtaga aagtgaagac ctgtgagaat
     2761 tctgcagtcg acggtaccgc gggcccggga tccaccggat ctagataact gatcataatc
     2821 agccatacca cattgtagag
//
