LOCUS       sequence-85483-e        1675 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1675)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-PCNA-19-NLS-4
FEATURES             Location/Qualifiers
     source          1..1675
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      14..23
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             38..58
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      71..80
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             77..784
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(222..240)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     490..509
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tcagaatccg ctagccacca tggcttcgtg gggctccccg aagaagaagc gcaaagtact
       61 ggtaccggtc gccaccatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga
      121 gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga
      181 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
      241 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa
      301 ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg
      361 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga
      421 ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc
      481 ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat
      541 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg
      601 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
      661 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat
      721 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta
      781 caagtccgga gaagggcaag ggcaagggca agggccgggc cgcggctacg cgtatcgatc
      841 catgttcgag gcgcgcctgg tccagggctc catcctcaag aaggtgttgg aggcactcaa
      901 ggacctcatc aacgaggcct gctgggatat tagctccagc ggtgtaaacc tgcagagcat
      961 ggactcgtcc cacgtctctt tggtgcagct caccctgcgg tctgagggct tcgacaccta
     1021 ccgctgcgac cgcaacctgg ccatgggcgt gaacctcacc agtatgtcca aaatactaaa
     1081 atgcgccggc aatgaagata tcattacact aagggccgaa gataacgcgg ataccttggc
     1141 gctagtattt gaagcaccaa accaggagaa agtttcagac tatgaaatga agttgatgga
     1201 tttagatgtt gaacaacttg gaattccaga acaggagtac agctgtgtag taaagatgcc
     1261 ttctggtgaa tttgcacgta tatgccgaga tctcagccat attggagatg ctgttgtaat
     1321 ttcctgtgca aaagacggag tgaaattttc tgcaagtgga gaacttggaa atggaaacat
     1381 taaattgtca cagacaagta atgtcgataa agaggaggaa gctgttacca tagagatgaa
     1441 tgaaccagtt caactaactt ttgcactgag gtacctgaac ttctttacaa aagccactcc
     1501 actctcttca acggtgacac tcagtatgtc tgcagatgta ccccttgttg tagagtataa
     1561 aattgcggat atgggacact taaaatacta cttggctccc aagatcgagg atgaagaagg
     1621 atcttaggga tccaccggat ctagataact gatcataatc agccatacca cattt
//
