LOCUS       sequence-85484-e        1704 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1704)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-PCNA-19-SV40NLS-4
FEATURES             Location/Qualifiers
     source          1..1704
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             29..49
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             53..73
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             77..97
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      104..113
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             110..817
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(255..273)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     523..542
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcca ccatggatcc aaaaaagaag agaaaggtag atccaaaaaa
       61 gaagagaaag gtagatccaa aaaagaagag aaaggtaccg gtcgccacca tggtgagcaa
      121 gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga
      181 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
      241 gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga
      301 catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat
      361 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
      421 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat
      481 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa
      541 gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg
      601 cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac
      661 cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt
      721 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg
      781 ccgccactcc accggcggca tggacgagct gtacaagtcc ggagaagggc aagggcaagg
      841 gcaagggccg ggccgcggct acgcgtatcg atccatgttc gaggcgcgcc tggtccaggg
      901 ctccatcctc aagaaggtgt tggaggcact caaggacctc atcaacgagg cctgctggga
      961 tattagctcc agcggtgtaa acctgcagag catggactcg tcccacgtct ctttggtgca
     1021 gctcaccctg cggtctgagg gcttcgacac ctaccgctgc gaccgcaacc tggccatggg
     1081 cgtgaacctc accagtatgt ccaaaatact aaaatgcgcc ggcaatgaag atatcattac
     1141 actaagggcc gaagataacg cggatacctt ggcgctagta tttgaagcac caaaccagga
     1201 gaaagtttca gactatgaaa tgaagttgat ggatttagat gttgaacaac ttggaattcc
     1261 agaacaggag tacagctgtg tagtaaagat gccttctggt gaatttgcac gtatatgccg
     1321 agatctcagc catattggag atgctgttgt aatttcctgt gcaaaagacg gagtgaaatt
     1381 ttctgcaagt ggagaacttg gaaatggaaa cattaaattg tcacagacaa gtaatgtcga
     1441 taaagaggag gaagctgtta ccatagagat gaatgaacca gttcaactaa cttttgcact
     1501 gaggtacctg aacttcttta caaaagccac tccactctct tcaacggtga cactcagtat
     1561 gtctgcagat gtaccccttg ttgtagagta taaaattgcg gatatgggac acttaaaata
     1621 ctacttggct cccaagatcg aggatgaaga aggatcttag ggatccaccg gatctagata
     1681 actgatcata atcagccata ccac
//
