LOCUS       sequence-85485-e        1965 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1965)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-PDHA1-N-10
FEATURES             Location/Qualifiers
     source          1..1965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1219..1228
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1225..1935
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1370..1388)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1638..1657
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gtcagatccg ctagcaccgc caccatgagg aagatgctcg ccgccgtctc ccgcgtgctg
       61 tctggcgctt ctcagaagcc ggcaagcaga gtgctggtag catcccgtaa ttttgcaaat
      121 gatgctacat ttgaaattaa gaaatgtgac cttcaccggc tggaagaagg ccctcctgtc
      181 acaacagtgc tcaccaggga ggatgggctc aaatactaca ggatgatgca gactgtacgc
      241 cgaatggagt tgaaagcaga tcagctgtat aaacagaaaa ttattcgtgg tttctgtcac
      301 ttgtgtgatg gtcaggaagc ttgctgtgtg ggcctggagg ccggcatcaa ccccacagac
      361 catctcatca cagcctaccg ggctcacggc tttactttca cccggggcct ttccgtccga
      421 gaaattctcg cagagcttac aggacgaaaa ggaggttgtg ctaaagggaa aggaggatcg
      481 atgcacatgt atgccaagaa cttctacggg ggcaatggca tcgtgggagc gcaggtgccc
      541 ctgggcgctg ggattgctct agcctgtaag tataatggaa aagatgaggt ctgcctgact
      601 ttatatggcg atggtgctgc taaccagggc cagatattcg aagcttacaa catggcagct
      661 ttgtggaaat taccttgtat tttcatctgt gagaataatc gctatggaat gggaacgtct
      721 gttgagagag cggcagccag cactgattac tacaagagag gcgatttcat tcctgggctg
      781 agagtggatg gaatggatat cctgtgcgtc cgagaggcaa caaggtttgc tgctgcctat
      841 tgtagatctg ggaaggggcc catcctgatg gagctgcaga cttaccgtta ccacggacac
      901 agtatgagtg accctggagt cagttaccgt acacgagaag aaattcagga agtaagaagt
      961 aagagtgacc ctattatgct tctcaaggac aggatggtga acagcaatct tgccagtgtg
     1021 gaagaactaa aggaaattga tgtggaagtg aggaaggaga ttgaggatgc tgcccagttt
     1081 gccacggccg atcctgagcc acctttggaa gagctgggct accacatcta ctccagcgac
     1141 ccaccttttg aagttcgtgg tgccaatcag tggatcaagt ttaagtcagt cagtagctcc
     1201 ggaggggatc caccggtcgc caccatggtg agcaagggcg aggaggataa catggccatc
     1261 atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc
     1321 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
     1381 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac
     1441 ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc
     1501 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg
     1561 acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc
     1621 aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc
     1681 gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg
     1741 aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg
     1801 cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac
     1861 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac
     1921 gagctgtaca agtaaagcgg ccgcgactct agatcataat cagcc
//
