LOCUS       sequence-85486-e        2625 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Centrosomes, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2625)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-PLK1-N-16
FEATURES             Location/Qualifiers
     source          1..2625
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1869..1878
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1875..2585
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2020..2038)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2288..2307
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gatccgctag cgccaccatg agtgctgcag tgactgcagg gaagctggca cgggcaccgg
       61 ccgaccctgg gaaagccggg gtccccggag ttgcagctcc cggagctccg gcggcggctc
      121 caccggcgaa agagatcccg gaggtcctag tggacccacg cagccggcgg cgctatgtgc
      181 ggggccgctt tttgggcaag ggcggctttg ccaagtgctt cgagatctcg gacgcggaca
      241 ccaaggaggt gttcgcgggc aagattgtgc ctaagtctct gctgctcaag ccgcaccaga
      301 gggagaagat gtccatggaa atatccattc accgcagcct cgcccaccag cacgtcgtag
      361 gattccacgg ctttttcgag gacaacgact tcgtgttcgt ggtgttggag ctctgccgcc
      421 ggaggtctct cctggagctg cacaagagga ggaaagccct gactgagcct gaggcccgat
      481 actacctacg gcaaattgtg cttggctgcc agtacctgca ccgaaaccga gttattcatc
      541 gagacctcaa gctgggcaac cttttcctga atgaagatct ggaggtgaaa ataggggatt
      601 ttggactggc aaccaaagtc gaatatgacg gggagaggaa gaagaccctg tgtgggactc
      661 ctaattacat agctcccgag gtgctgagca agaaagggca cagtttcgag gtggatgtgt
      721 ggtccattgg gtgtatcatg tataccttgt tagtgggcaa accacctttt gagacttctt
      781 gcctaaaaga gacctacctc cggatcaaga agaatgaata cagtattccc aagcacatca
      841 accccgtggc cgcctccctc atccagaaga tgcttcagac agatcccact gcccgcccaa
      901 ccattaacga gctgcttaat gacgagttct ttacttctgg ctatatccct gcccgtctcc
      961 ccatcacctg cctgaccatt ccaccaaggt tttcgattgc tcccagcagc ctggacccca
     1021 gcaaccggaa gcccctcaca gtcctcaata aaggcttgga gaaccccctg cctgagcgtc
     1081 cccgggaaaa agaagaacca gtggttcgag agacaggtga ggtggtcgac tgccacctca
     1141 gtgacatgct gcagcagctg cacagtgtca atgcctccaa gccctcggag cgtgggctgg
     1201 tcaggcaaga ggaggctgag gatcctgcct gcatccccat cttctgggtc agcaagtggg
     1261 tggactattc ggacaagtac ggccttgggt atcagctctg tgataacagc gtgggggtgc
     1321 tcttcaatga ctcaacacgc ctcatcctct acaatgatgg tgacagcctg cagtacatag
     1381 agcgtgacgg cactgagtcc tacctcaccg tgagttccca tcccaactcc ttgatgaaga
     1441 agatcaccct ccttaaatat ttccgcaatt acatgagcga gcacttgctg aaggcaggtg
     1501 ccaacatcac gccgcgcgaa ggtgatgagc tcgcccggct gccctaccta cggacctggt
     1561 tccgcacccg cagcgccatc atcctgcacc tcagcaacgg cagcgtgcag atcaacttct
     1621 tccaggatca caccaagctc atcttgtgcc cactgatggc agccgtgacc tacatcgacg
     1681 agaagcggga cttccgcaca taccgcctga gtctcctgga ggagtacggc tgctgcaagg
     1741 agctggccag ccggctccgc tacgcccgca ctatggtgga caagctgctg agctcacgct
     1801 cggccagcaa ccgtctcaag gcctccggta gcggcagcgc atccggcgga agcggaagcg
     1861 gaccggtcgc caccatggtg agcaagggcg aggaggataa catggccatc atcaaggagt
     1921 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg
     1981 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
     2041 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg
     2101 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct
     2161 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact
     2221 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
     2281 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt
     2341 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg
     2401 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg
     2461 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg
     2521 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca
     2581 agtaaagcgg ccgcgactct agatcataat cagccatacc acatt
//
