LOCUS       sequence-85487-e        1401 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Peroxisomes, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1401)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1401)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-PMP-N-10
FEATURES             Location/Qualifiers
     source          1..1401
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      30..39
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      645..654
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             651..1361
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(796..814)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1064..1083
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gatccgctag cgctaccgga ctcagatctg ccaccatggc gccggccgcg tccaggctgc
       61 gggccgaagc cgggctcggg gcgctgccgc ggcgggcgct cgcccagtac ttgctcttcc
      121 tgcggctcta cccggtgctc accaaggcgg ccaccagtgg cattttgtca gcacttggga
      181 acttcctggc ccagatgatt gagaagaagc ggaaaaaaga aaactctaga agtctggatg
      241 tcggtgggcc tctgagatat gccgtttacg ggttcttctt cacagggccg ctgagtcact
      301 tcttctactt cttcatggaa cattggatcc ctcctgaggt ccccctggca gggctcagga
      361 ggcttctcct ggaccgcctc gtctttgcac cggccttcct catgttgttc ttcctcatca
      421 tgaactttct ggaggggaaa gacgcctcag ccttcgccgc caagatgagg gggggcttct
      481 ggccggcgct gaggatgaac tggcgggtgt ggacgccact acagttcatc aacatcaact
      541 acgtccctct gaagttccgg gtgctcttcg ccaacctggc agctctgttc tggtatgcct
      601 acctggcctc cttggggaag agcggctccg gtagcggacc ggtcgccacc atggtgagca
      661 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
      721 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
      781 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
      841 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
      901 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
      961 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
     1021 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
     1081 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     1141 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     1201 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     1261 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     1321 gccgccactc caccggcggc atggacgagc tgtacaagta aagcggccgc gactctagat
     1381 cataatcagc cataccacat t
//
