LOCUS       sequence-85489-e        3976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesion Kinase, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3976)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 3976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-PTK2-C-10
FEATURES             Location/Qualifiers
     source          1..3976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      21..30
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             27..734
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(172..190)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     440..459
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggaggat aacatggcca
       61 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt
      121 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
      181 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt
      241 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct
      301 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg
      361 tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca
      421 ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct
      481 ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc
      541 tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg
      601 tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg
      661 actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg
      721 acgagctgta caagtccgga ctcagatcta gcggtggcag tggagcagct gcttaccttg
      781 accccaactt gaatcacaca ccaaattcga gtactaagac tcacctgggt actggtatgg
      841 aacgttctcc tggtgcaatg gagcgagtat taaaggtctt tcattatttt gaaagcaata
      901 gtgagccaac cacctgggcc agtattatca ggcatggaga tgctactgat gtcaggggca
      961 tcattcagaa gatagtggac agtcacaaag taaagcatgt ggcctgctat ggattccgcc
     1021 tcagtcacct gcggtcagag gaggttcact ggcttcacgt ggatatgggc gtctccagtg
     1081 tgagggagaa gtatgagctt gctcacccac cagaggagtg gaaatatgaa ttgagaattc
     1141 gttatttgcc aaaaggattt ctaaaccagt ttactgaaga taagccaact ttgaatttct
     1201 tctatcaaca ggtgaagagc gattatatgt tagagatagc tgatcaagtg gaccaggaaa
     1261 ttgctttgaa gttgggttgt ctagaaatac ggcgatcata ctgggagatg cggggcaatg
     1321 cactagaaaa gaagtctaac tatgaagtat tagaaaaaga tgttggttta aagcgatttt
     1381 ttcctaagag tttactggat tctgtcaagg ccaaaacact aagaaaactg atccaacaaa
     1441 catttagaca atttgccaac cttaatagag aagaaagtat tctgaaattc tttgagatcc
     1501 tgtctccagt ctacagattt gataaggaat gcttcaagtg tgctcttggt tcaagctgga
     1561 ttatttcagt ggaactggca atcggcccag aagaaggaat cagttaccta acggacaagg
     1621 gctgcaatcc cacacatctt gctgacttca ctcaagtgca aaccattcag tattcaaaca
     1681 gtgaagacaa ggacagaaaa ggaatgctac aactaaaaat agcaggtgca cccgagcctc
     1741 tgacagtgac ggcaccatcc ctaaccattg cggagaatat ggctgaccta atagatgggt
     1801 actgccggct ggtgaatgga acctcgcagt catttatcat cagacctcag aaagaaggtg
     1861 aacgggcttt gccatcaata ccaaagttgg ccaacagcga aaagcaaggc atgcggacac
     1921 acgccgtctc tgtgtcagaa acagatgatt atgctgagat tatagatgaa gaagatactt
     1981 acaccatgcc ctcaaccagg gattatgaga ttcaaagaga aagaatagaa cttggacgat
     2041 gtattggaga aggccaattt ggagatgtac atcaaggcat ttatatgagt ccagagaatc
     2101 cagctttggc ggttgcaatt aaaacatgta aaaactgtac ttcggacagc gtgagagaga
     2161 aatttcttca agaagcctta acaatgcgtc agtttgacca tcctcatatt gtgaagctga
     2221 ttggagtcat cacagagaat cctgtctgga taatcatgga gctgtgcaca cttggagagc
     2281 tgaggtcatt tttgcaagta aggaaataca gtttggatct agcatctttg atcctgtatg
     2341 cctatcagct tagtacagct cttgcatatc tagagagcaa aagatttgta cacagggaca
     2401 ttgctgctcg gaatgttctg gtgtcctcaa atgattgtgt aaaattagga gactttggat
     2461 tatcccgata tatggaagat agtacttact acaaagcttc caaaggaaaa ttgcctatta
     2521 aatggatggc tccagagtca atcaattttc gacgttttac ctcagctagt gacgtatgga
     2581 tgtttggtgt gtgtatgtgg gagatactga tgcatggtgt gaagcctttt caaggagtga
     2641 agaacaatga tgtaatcggt cgaattgaaa atggggaaag attaccaatg cctccaaatt
     2701 gtcctcctac cctctacagc cttatgacga aatgctgggc ctatgacccc agcaggcggc
     2761 ccaggtttac tgaacttaaa gctcagctca gcacaatcct ggaggaagag aaggctcagc
     2821 aagaagagcg catgaggatg gagtccagaa gacaggccac agtgtcctgg gactccggag
     2881 ggtctgatga agcaccgccc aagcccagca gaccgggtta tcccagtccg aggtccagcg
     2941 aaggatttta tcccagccca cagcacatgg tacaaaccaa tcattaccag gtttctggct
     3001 accctggttc acatggaatc acagccatgg ctggcagcat ctatccaggt caggcatctc
     3061 ttttggacca aacagattca tggaatcata gacctcagga gatagcaatg tggcagccca
     3121 atgtggagga ctctacagta ttggacctgc gagggattgg gcaagtgttg ccaacccatc
     3181 tgatggaaga gcgtctaatc cgacagcaac aggaaatgga agaagatcag cgctggctgg
     3241 aaaaagagga aagatttctg aaacctgatg tgagactctc tcgaggcagt attgacaggg
     3301 aggatggaag tcttcagggt ccgattggaa accaacatat atatcagcct gtgggtaaac
     3361 cagatcctgc agctccacca aagaaaccgc ctcgccctgg agctcccggt catctgggaa
     3421 gccttgccag cctcagcagc cctgctgaca gctacaacga gggtgtcaag ccatggaggc
     3481 ttcagcccca ggaaatcagc ccccctccta ctgccaacct ggaccggtcg aatgataagg
     3541 tgtacgagaa tgtgacgggc ctggtgaaag ctgtcatcga gatgtccagt aaaatccagc
     3601 cagccccacc agaggagtat gtccctatgg tgaaggaagt cggcttggcc ctgaggacat
     3661 tattggccac tgtggatgag accattcccc tcctaccagc cagcacccac cgagagattg
     3721 agatggcaca gaagctattg aactctgacc tgggtgagct catcaacaag atgaaactgg
     3781 cccagcagta tgtcatgacc agcctccagc aagagtacaa aaagcaaatg ctgactgctg
     3841 ctcacgccct ggctgtggat gccaaaaact tactcgatgt cattgaccaa gcaagactga
     3901 aaatgcttgg gcagacgaga ccacactgag gatccaccgg atctagataa ctgatcataa
     3961 tcagccatac cacatt
//
