LOCUS       sequence-85491-e        1459 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Ras GTPase, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1459)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Rab11a-7
FEATURES             Location/Qualifiers
     source          1..1459
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..742
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(180..198)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     448..467
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggaggataa
       61 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
      121 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
      181 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
      241 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
      301 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
      361 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
      421 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
      481 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag
      541 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa
      601 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca
      661 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
      721 cggcatggac gagctgtaca agtccggact cagatctcga gccatgggca cccgcgacga
      781 cgagtacgac tacctcttta aagttgtcct tattggagat tctggtgttg gaaagagtaa
      841 tctcctgtct cgatttactc gaaatgagtt taatctggaa agcaagagca ccattggagt
      901 agagtttgca acaagaagca tccaggttga tggaaaaaca ataaaggcac agatatggga
      961 cacagcaggg caagagcgat atcgagctat aacatcagca tattatcgtg gagctgtagg
     1021 tgccttattg gtttatgaca ttgctaaaca tctcacatat gaaaatgtag agcgatggct
     1081 gaaagaactg agagatcatg ctgatagtaa cattgttatc atgcttgtgg gcaataagag
     1141 tgatctacgt catctcaggg cagttcctac agatgaagca agagcttttg cagaaaagaa
     1201 tggtttgtca ttcattgaaa cttcggccct agactctaca aatgtagaag ctgcttttca
     1261 gacaatttta acagagattt accgcattgt ttctcagaag caaatgtcag acagacgcga
     1321 aaatgacatg tctccaagca acaatgtggt tcctattcat gttccaccaa ccactgaaaa
     1381 caagccaaag gtgcagtgct gtcagaacat ctaaggatcc accggatcta gataactgat
     1441 cataatcagc cataccaca
//
