LOCUS       sequence-85492-e        1464 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Ras GTPase, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1464)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1464)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Rab4a-7
FEATURES             Location/Qualifiers
     source          1..1464
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             33..740
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(178..196)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     446..465
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cgtcagatcc gctagcgcta ccggtcgcca ccatggtgag caagggcgag gaggataaca
       61 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc
      121 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
      181 agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt
      241 tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc
      301 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg
      361 tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc
      421 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg
      481 cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc
      541 tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga
      601 agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca
      661 acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg
      721 gcatggacga gctgtacaag tccggactca gatctcgagc catgtcgcag acggccatgt
      781 ccgaaaccta cgattttttg tttaagttct tggttattgg aaatgcagga actggcaaat
      841 cttgcttact tcatcagttt attgaaaaaa aattcaaaga tgactcaaat catacaatag
      901 gagtggaatt tggttcaaag ataataaatg ttggtggtaa atatgtaaag ttacaaatat
      961 gggatacagc aggacaagaa cgattcaggt ccgtgacgag aagttattac cgaggcgcgg
     1021 ccggggctct cctcgtctat gatatcacca gccgagaaac ctacaatgcg cttactaatt
     1081 ggttaacaga tgcccgaatg ctggcgagcc agaacattgt gatcatcctt tgtggaaaca
     1141 agaaggacct ggatgcagat cgtgaagtta ccttcttaga agcctccaga tttgctcaag
     1201 aaaatgagct gatgtttttg gaaacaagtg cgctcacagg ggagaatgta gaagaggctt
     1261 ttgtacagtg tgcaagaaaa atacttaaca aaatcgaatc aggtgagctg gacccagaaa
     1321 gaatgggctc aggtattcag tacggagatg ctgccttgag acagctgagg tcaccgcggc
     1381 gcgcacaggc cccgaacgct caggagtgtg gttgttaggg atccaccgga tctagataac
     1441 tgatcataat cagccatacc acat
//
