LOCUS       sequence-85494-e        1420 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Ras GTPase, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1420)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1420)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Rab7a-7
FEATURES             Location/Qualifiers
     source          1..1420
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..741
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(179..197)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     447..466
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggaggataac
       61 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
      121 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
      181 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
      241 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
      301 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
      361 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
      421 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
      481 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
      541 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
      601 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
      661 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
      721 ggcatggacg agctgtacaa gtccggactc agatctcgag ccatgacctc taggaagaaa
      781 gtgttgctga aggttatcat cctgggagat tctggagtcg ggaagacatc actcatgaac
      841 cagtatgtga ataagaaatt cagcaatcag tacaaagcca caataggagc tgactttctg
      901 accaaggagg tgatggtgga tgacaggcta gtcacaatgc agatatggga cacagcagga
      961 caggaacggt tccagtctct cggtgtggcc ttctacagag gtgcagactg ctgcgttctg
     1021 gtatttgatg tgactgcccc caacacattc aaaaccctag atagctggag agatgagttt
     1081 ctcatccagg ccagtccccg agatcctgaa agcttcccat ttgttgtgtt gggaaacaag
     1141 attgacctcg aaaacagaca agtggccaca aagcgggcac aggcctggtg ctacagcaaa
     1201 aacaacattc cctactttga gaccagtgcc aaggaggcca tcaacgtgga gcaggcgttc
     1261 cagacgattg cacggaatgc acttaagcag gaaacggagg tggagctgta caacgaattt
     1321 cctgaaccta tcaaactgga caagaatgac cgggccaagg cctcggcaga aagctgcagt
     1381 tgctgaggat ccaccggatc tagataactg atcataatca
//
