LOCUS       sequence-85495-e        1349 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubule-Binding Protein, Excitation: 587, 
            Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1349)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-RatLC3B-C-7
FEATURES             Location/Qualifiers
     source          1..1349
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             26..733
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(171..189)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     439..458
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             755..1177
                     /codon_start=1
                     /gene="Map1lc3b"
                     /product="rat LC3 protein"
                     /label=LC3
                     /note="Atg8 family member found on autophagosomes"
                     /translation="PSEKTFKQRRSFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLPV
                     LDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESERDE
                     DGFLYMVYASQETFGTALAVTYMSALKATATGREPCL"
ORIGIN
        1 tccgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggaggata acatggccat
       61 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt
      121 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
      181 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta
      241 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt
      301 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt
      361 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac
      421 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc
      481 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct
      541 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt
      601 gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga
      661 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga
      721 cgagctgtac aagtccggac tcagatcctc tagaccgtcc gagaagacct tcaaacagcg
      781 ccggagcttc gaacaaagag tggaagatgt ccggctcatc cgggagcagc accccaccaa
      841 gatcccagtg attatagagc gatacaaggg tgagaagcag ctgcccgtcc tggacaagac
      901 caagttcctt gtacctgatc acgtgaatat gagcgaactc atcaagataa ttagaaggcg
      961 cctgcagctc aatgctaacc aagccttctt cctcctggtg aatgggcaca gcatggtgag
     1021 tgtgtccaca cccatctctg aagtgtacga gagcgagaga gatgaagacg gcttcctgta
     1081 catggtctat gcctcccagg agacgttcgg gacagcactg gctgttacat acatgtcagc
     1141 tctgaaggca acagcaacag gaagagagcc atgcttgtga cagacataca gccacttcca
     1201 actaaagcaa gcctctgctc cctgctacct gcatggagcc cactgtgaca ctcagaccat
     1261 ccccggtcac tcactcactc gtgtctgaga atctcagtga gagctgcctc tgtcacggac
     1321 ggaagccaac acagccacct ctcgacctg
//
