LOCUS       sequence-85498-e        1934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Scaffold, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1934)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Sequestosome1-C-18
FEATURES             Location/Qualifiers
     source          1..1934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..739
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(177..195)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     445..464
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gtcagatccg ctagcgctac cggtcgccac catggtgagc aagggcgagg aggataacat
       61 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca
      121 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
      181 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt
      241 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct
      301 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
      361 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg
      421 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
      481 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct
      541 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa
      601 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa
      661 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg
      721 catggacgag ctgtacaagt ccggactcag atctcgagag agcggaggta gcgcatccgg
      781 cggaagcgga ggagccatgt cctacgtgaa ggatgacatc ttccgaatct acattaaaga
      841 gaaaaaagag tgccggcggg accaccgccc accgtgtgct caggaggcgc cccgcaacat
      901 ggtgcacccc aatgtgatct gcgatggctg caatgggcct gtggtaggaa cccgctacaa
      961 gtgcagcgtc tgcccagact acgacttgtg tagcgtctgc gagggaaagg gcttgcaccg
     1021 ggggcacacc aagctcgcat tccccagccc cttcgggcac ctgtctgagg gcttctcgca
     1081 cagccgctgg ctccggaagg tgaaacacgg acacttcggg tggccaggat gggaaatggg
     1141 tccaccagga aactggagcc cacgtcctcc tcgtgcaggg gaggcccgcc ctggccccac
     1201 ggcagaatca gcttctggtc catcggagga tccgagtgtg aatttcctga agaacgttgg
     1261 ggagagtgtg gcagctgccc ttagccctct gggcattgaa gttgatatcg atgtggagca
     1321 cggagggaaa agaagccgcc tgacccccgt ctctccagag agttccagca cagaggagaa
     1381 gagcagctca cagccaagca gctgctgctc tgatcccagc aagccgggtg ggaatgttga
     1441 gggcgccacg cagtctctgg cggagcagat gagaaagatc gccttggagt ccgaggggcg
     1501 ccctgaggaa cagatggagt cggataactg ttcaggagga gatgatgact ggacccatct
     1561 gtcttcaaaa gaagtggacc cgtctacagg tgaactccag tccctacaga tgccagaatc
     1621 cgaagggcca agctctctgg acccctccca ggagggaccc acagggctga aggaagctgc
     1681 cttgtaccca catctcccgc cagaggctga cccgcggctg attgagtccc tctcccagat
     1741 gctgtccatg ggcttctctg atgaaggcgg ctggctcacc aggctcctgc agaccaagaa
     1801 ctatgacatc ggagcggctc tggacaccat ccagtattca aagcatcccc cgccgttgtg
     1861 agaattctgc agtcgacggt accgcgggcc cgggatccac cggatctaga taactgatca
     1921 taatcagcca tacc
//
