LOCUS       sequence-85499-e        1882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Scaffold, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1882)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Sequestosome1-N-18
FEATURES             Location/Qualifiers
     source          1..1882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1129..1138
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1135..1845
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1280..1298)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1548..1567
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gctagcgcca ccatggccat gtcctacgtg aaggatgaca tcttccgaat ctacattaaa
       61 gagaaaaaag agtgccggcg ggaccaccgc ccaccgtgtg ctcaggaggc gccccgcaac
      121 atggtgcacc ccaatgtgat ctgcgatggc tgcaatgggc ctgtggtagg aacccgctac
      181 aagtgcagcg tctgcccaga ctacgacttg tgtagcgtct gcgagggaaa gggcttgcac
      241 cgggggcaca ccaagctcgc attccccagc cccttcgggc acctgtctga gggcttctcg
      301 cacagccgct ggctccggaa ggtgaaacac ggacacttcg ggtggccagg atgggaaatg
      361 ggtccaccag gaaactggag cccacgtcct cctcgtgcag gggaggcccg ccctggcccc
      421 acggcagaat cagcttctgg tccatcggag gatccgagtg tgaatttcct gaagaacgtt
      481 ggggagagtg tggcagctgc ccttagccct ctgggcattg aagttgatat cgatgtggag
      541 cacggaggga aaagaagccg cctgaccccc gtctctccag agagttccag cacagaggag
      601 aagagcagct cacagccaag cagctgctgc tctgatccca gcaagccggg tgggaatgtt
      661 gagggcgcca cgcagtctct ggcggagcag atgagaaaga tcgccttgga gtccgagggg
      721 cgccctgagg aacagatgga gtcggataac tgttcaggag gagatgatga ctggacccat
      781 ctgtcttcaa aagaagtgga cccgtctaca ggtgaactcc agtccctaca gatgccagaa
      841 tccgaagggc caagctctct ggacccctcc caggagggac ccacagggct gaaggaagct
      901 gccttgtacc cacatctccc gccagaggct gacccgcggc tgattgagtc cctctcccag
      961 atgctgtcca tgggcttctc tgatgaaggc ggctggctca ccaggctcct gcagaccaag
     1021 aactatgaca tcggagcggc tctggacacc atccagtatt caaagcatcc cccgccgttg
     1081 agcagcggtg gagcaagcgc agccagtggt agcgcagcgg gaccggtcgc caccatggtg
     1141 agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac
     1201 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
     1261 tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc
     1321 tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc
     1381 gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
     1441 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag
     1501 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag
     1561 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg
     1621 aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc
     1681 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc
     1741 aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc
     1801 gagggccgcc actccaccgg cggcatggac gagctgtaca agtaaagcgg ccgcgactct
     1861 agatcataat cagccatacc ac
//
