LOCUS       sequence-85500-e         980 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Golgi TGN, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 980)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-SiT-N-15
FEATURES             Location/Qualifiers
     source          1..980
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      223..232
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             229..939
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(374..392)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     642..661
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggactca gatctcgagc tcgccaccat gattcacacc
       61 aacctgaaga aaaagttcag ctgctgcgtc ctggtctttc ttctgtttgc agtcatctgt
      121 gtgtggaagg aaaagaagaa agggagttac tatgattcct ttaaattgca aaccaaggaa
      181 ttcctgcagt cgacggtacc gcgggcccgg gatccaccgg tcgccaccat ggtgagcaag
      241 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
      301 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
      361 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
      421 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
      481 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
      541 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
      601 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
      661 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
      721 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
      781 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg
      841 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
      901 cgccactcca ccggcggcat ggacgagctg tacaagtaaa gcggccgcga ctctagatca
      961 taatcagcca taccacattt
//
