LOCUS       sequence-85502-e        2127 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cilium, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2127)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-SSTR3-N-17
FEATURES             Location/Qualifiers
     source          1..2127
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1370..1379
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1376..2086
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1521..1539)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1789..1808
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 agatccgcta gcgctaccgg actcagatct cgagaccgcc atggccactg ttacctatcc
       61 ttcatctgag cctacgacct tggaccctgg gaacgcatcc tcgacctggc ccctggatac
      121 caccctgggg aacacatccg ctggcgctag cctgacaggc ctggctgtca gtggcatctt
      181 gatctctctg gtgtacctgg tggtgtgcgt ggtgggtttg ctgggcaact cgctggtgat
      241 ctacgtggtc ctgcggcaca cgtccagccc atcagtgacc agtgtctata tcctcaacct
      301 ggctctggct gatgagctct tcatgctagg gctacccttc ctggctgctc agaacgccct
      361 gtcctactgg cccttcggat ctctcatgtg ccgtctggtc atggccgtgg atggcatcaa
      421 ccagttcacc agcatcttct gcctcaccgt catgagtgtg gaccgctatc tggctgtggt
      481 gcaccccaca cgctcagccc gctggcgcac ggcaccagtg gctcgcacgg tcagtgcagc
      541 tgtctgggtg gcctcggctg tggtggtgct gcctgtggtt gtgttctcag gagtgccccg
      601 gggcatgagc acgtgccaca tgcagtggcc agagccagcg gctgcctggc gaacagcctt
      661 catcatctac acggccgcac tgggcttctt tgggcccctg ctggtcatct gcttgtgcta
      721 cttgctcatt gtggtaaagg tgcggtcgac cacccggcgg gtgcgggcgc cctcgtgtca
      781 gtgggtacag gcacccgcat gccagcggcg acgccgctct gagcgcaggg tcacacgcat
      841 ggtggtggcc gtggtggcac tcttcgtcct ctgctggatg cctttctatc tgctcaacat
      901 cgtcaatgtg gtgtgcccgc tgccggagga gcccgccttc ttcggcctct acttcctggt
      961 ggtggcgctg ccctacgcca acagctgcgc aaaccccatc ctctacggct tcctctccta
     1021 ccgcttcaag cagggctttc gcaggatcct gctaagacca tcacgtcgca ttcggagcca
     1081 ggagccaggg tcgggacctc cagagaagac tgaagaggag gaggatgaag aagaagaaga
     1141 gagaagggaa gaggaggagc ggaggatgca gagagggcag gagatgaacg ggaggctcag
     1201 tcagatcgca caggctggca ctagtggaca acagccacgg ccctgcacag ggactgctaa
     1261 ggagcagcag cttctgcccc aggaggccac agctggggac aaggccagca cactgagcca
     1321 tctgggaatt ctgcagtcga cggtaccgcg ggcccgggat ccaccggtcg ccaccatggt
     1381 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca
     1441 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     1501 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
     1561 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc
     1621 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     1681 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga
     1741 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
     1801 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
     1861 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
     1921 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
     1981 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
     2041 cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtaaagcg gccgcgactc
     2101 tagatcataa tcagccatac cacattt
//
